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Automatic annular laser trapping: a system for high-throughput sperm analysis and sorting.

Linda Shi1, Bing Shao, Timothy Chen

  • 1UCI - Beckman Laser Institute, 1002 Health Sciences Road, Irvine, CA 92612, USA. zhixiashi@gmail.com

Journal of Biophotonics
|April 4, 2009
PubMed
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This study presents an automated microscope system for analyzing sperm motility using an annular laser trap. The system enables parallel sorting and comparison of sperm from different species based on their swimming speed.

Area of Science:

  • Biomedical Engineering
  • Reproductive Biology
  • Optical Physics

Background:

  • Sperm motility is crucial for natural fertilization.
  • Current methods for analyzing sperm motility can be time-consuming and lack high-throughput capabilities.
  • Laser-based manipulation offers potential for precise control and analysis of microscopic samples.

Purpose of the Study:

  • To develop and validate an automatic microscope system for studying sperm motility.
  • To utilize a continuous annular laser trap for parallel analysis and sorting of sperm.
  • To investigate the effects of laser radiation and optical forces on sperm behavior.

Main Methods:

  • An automated microscope system was designed integrating a phase contrast microscope with a continuous annular laser trap.

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  • Sperm images were digitized at video rates, and the microscope stage was controlled in real-time for sperm relocation.
  • Sperm tracking and swimming behavior identification were performed automatically.
  • Parallel sorting of human and gorilla sperm was achieved using the system.

    • Main Results:

    • The system successfully enabled parallel analysis and sorting of sperm based on motility.
    • Threshold power levels differentiating fast and slow sperm groups were determined for human and gorilla sperm.
    • The system demonstrated the capability to study sperm response to laser radiation and optical forces.

    Conclusions:

    • The developed automatic microscope system provides an efficient platform for high-throughput sperm analysis and sorting.
    • This technology allows for detailed investigation into sperm motility and its response to optical manipulation.
    • Comparative analysis of sperm motility characteristics between species is facilitated by this system.