Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Hybrid Zones02:29

Hybrid Zones

Hybrid zones are narrow regions where two closely related species interact, mate, and produce hybrids. Relative to either parent species, hybrids may possess distinct phenotypic or genetic differences that impact their survival and reproductive success. The genetic variances introduced by hybridization influence species diversity and speciation processes within the hybrid zone.Gene flow and natural selection are evolutionary mechanisms that shape the outcome of a hybrid zone. Gene flow...
Hybridoma Technology01:31

Hybridoma Technology

Hybridoma technology is used for the large-scale production of monoclonal antibodies. Monoclonal antibodies bind to only a single antigenic determinant or epitope. Such antibodies are used in research, diagnostics, and disease therapy. The hybridoma technology established in 1975 by Georges Köhler and Cesar Milstein was awarded the Nobel Prize in Medicine in 1984 for revolutionizing research and therapy.
Hybridoma Selection
Commonly used fusion techniques — electroporation, polyethylene glycol...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Widespread siderophore production among Symbiodiniaceae-associated bacteria.

FEMS microbiology letters·2026
Same author

The MHV-68 nuclear egress complex supports C-capsid selective capsid egress.

Journal of virology·2026
Same author

Mitral valve replacement in a case of dextrocardia with situs solitus.

JTCVS techniques·2026
Same author

H2A.Z and H3:K56Q Affect Transcription Through Chromatin and Yeast FACT-Dependent Nucleosome Unfolding.

International journal of molecular sciences·2025
Same author

Diffuse infiltrating retinoblastoma: a multicentre, international, data-sharing study.

The British journal of ophthalmology·2025
Same author

Poly(ADP-ribose)polymerase 2 is zinc-dependent enzyme and nucleosome reorganizer.

Cellular and molecular life sciences : CMLS·2025
Same journal

Nondenaturing Polyacrylamide Gel Electrophoresis: Preparation and Analysis of DNA.

Current protocols in molecular biology·2021
Same journal

Purification and Concentration of DNA from Aqueous Solutions: Preparation and Analysis of DNA.

Current protocols in molecular biology·2021
Same journal

Expression of Proteins Using Semliki Forest Virus Vectors: Protein Expression.

Current protocols in molecular biology·2021
Same journal

Methylation and Uracil Interference Assays for Analysis of Protein-DNA Interactions: DNA-Protein Interactions.

Current protocols in molecular biology·2021
Same journal

Separation of Double- and Single-Stranded Nucleic Acids Using Hydroxylapatite Chromatography: Preparation and Analysis of DNA.

Current protocols in molecular biology·2021
Same journal

Pulsed-Field Gel Electrophoresis: Preparation and Analysis of DNA.

Current protocols in molecular biology·2021
See all related articles

Related Experiment Video

Updated: Jun 24, 2026

A Yeast 2-Hybrid Screen in Batch to Compare Protein Interactions
14:23

A Yeast 2-Hybrid Screen in Batch to Compare Protein Interactions

Published on: June 6, 2018

Two-hybrid dual bait system.

Elena Kotova1, Thomas Coleman, Ilya Serebriiskii

  • 1Fox Chase Cancer Center, Philadelphia, Pennsylvania, USA.

Current Protocols in Molecular Biology
|April 4, 2009
PubMed
Summary
This summary is machine-generated.

The dual bait yeast two-hybrid system enables comparing two proteins against one prey simultaneously. This versatile method aids in identifying and confirming protein-protein interactions efficiently.

More Related Videos

Split-Ubiquitin Based Membrane Yeast Two-Hybrid (MYTH) System: A Powerful Tool For Identifying Protein-Protein Interactions
14:04

Split-Ubiquitin Based Membrane Yeast Two-Hybrid (MYTH) System: A Powerful Tool For Identifying Protein-Protein Interactions

Published on: February 1, 2010

A Novel Method of Drug Administration to Multiple Zebrafish (Danio rerio) and the Quantification of Withdrawal
10:52

A Novel Method of Drug Administration to Multiple Zebrafish (Danio rerio) and the Quantification of Withdrawal

Published on: November 12, 2014

Related Experiment Videos

Last Updated: Jun 24, 2026

A Yeast 2-Hybrid Screen in Batch to Compare Protein Interactions
14:23

A Yeast 2-Hybrid Screen in Batch to Compare Protein Interactions

Published on: June 6, 2018

Split-Ubiquitin Based Membrane Yeast Two-Hybrid (MYTH) System: A Powerful Tool For Identifying Protein-Protein Interactions
14:04

Split-Ubiquitin Based Membrane Yeast Two-Hybrid (MYTH) System: A Powerful Tool For Identifying Protein-Protein Interactions

Published on: February 1, 2010

A Novel Method of Drug Administration to Multiple Zebrafish (Danio rerio) and the Quantification of Withdrawal
10:52

A Novel Method of Drug Administration to Multiple Zebrafish (Danio rerio) and the Quantification of Withdrawal

Published on: November 12, 2014

Area of Science:

  • Molecular Biology
  • Biochemistry
  • Genetics

Background:

  • Protein-protein interactions are crucial for cellular functions.
  • The yeast two-hybrid system is a widely used method for identifying these interactions.
  • Adaptations of the system enhance its utility and efficiency.

Purpose of the Study:

  • To introduce and describe the dual bait yeast two-hybrid system.
  • To highlight its capability for simultaneous comparison of two baits with one prey.
  • To explain its application in identifying protein-protein interactions.

Main Methods:

  • Utilizing a dual bait system with LexA and cI DNA-binding domain fusions.
  • Employing yeast strains engineered with four separate reporter genes.
  • Expressing a prey protein fused to an activation domain for transcriptional activation.

Main Results:

  • The dual bait system allows for the simultaneous screening of two distinct bait proteins against a single prey.
  • This method facilitates the direct comparison of interaction potentials.
  • It enables the identification of protein interactors from cDNA libraries or defined proteins.

Conclusions:

  • The dual bait yeast two-hybrid system is a powerful adaptation for studying protein-protein interactions.
  • It offers a streamlined approach for comparative interaction analysis.
  • This technique enhances the versatility and efficiency of interaction discovery in molecular biology research.