Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Mass Spectrometers01:16

Mass Spectrometers

This lesson details the instrumentation of a mass spectrometer—a physical instrument to perform mass spectrometry on analyte molecules and record the characteristic mass spectra. This is achieved via three chief functions:
MALDI-TOF Mass Spectrometry01:19

MALDI-TOF Mass Spectrometry

Mass spectrometry is a powerful characterization technique that can identify and separate a wide variety of compounds ranging from chemical to biological entities, based on their mass-to-charge ratio (m/z). The instruments that allow this detection, known as mass spectrometers, have three components: an ion source, a mass analyzer, and a detector. These spectrometers differ based on the nature of their ion source and analyzers.Matrix-assisted laser desorption ionization (MALDI) is a commonly...
Mass Spectrometry: Complex Analysis01:21

Mass Spectrometry: Complex Analysis

Mass spectrometry is an important technique for the identification of pure compounds. However, it has some limitations for the analysis of complex mixtures, often due to excessive fragmentation making the spectrum too complicated to decipher. Mass spectrometry can be combined with suitable separation methods in sequence, forming hyphenated methods, which are useful in the analysis of complex mixtures.
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...
Mass Spectrometry: Overview01:19

Mass Spectrometry: Overview

Mass spectrometry is an analytical technique used to determine the molecular mass and molecular formula of a compound. The basic principle of mass spectrometry is to generate ions from the analyte molecule and measure these ion abundances against their molecular mass. One common type of ionization, known as electron ionization or EI, bombards the analyte molecules in the gas phase with high-energy electron beams. The electron beams displace an electron from the molecule and leave behind a...
Mass Analyzers: Overview01:13

Mass Analyzers: Overview

The mass analyzer is a crucial component of the mass spectrometer. In the ionization chamber, the vaporized sample is bombarded with a high-energy electron beam to generate a radical cation and further fragment into neutral molecules, radicals, and cations. A series of negatively charged accelerator plates accelerate the cations into the mass analyzer. The mass analyzer separates ions according to their mass-to-charge (m/z) ratios and then directs them to the detector. The common types of mass...
Tandem Mass Spectrometry01:21

Tandem Mass Spectrometry

Tandem mass spectrometry is a technique that uses multiple mass analyzers in series to obtain a higher selectivity and reduce chemical noise during analyte detection. Instruments with multiple analyzers separated by an interaction cell enable secondary fragmentation and selected study of the fragment ions.Secondary fragmentations occur in the interaction cell and can be induced by various factors. Fragmentation induced by collision with inert gases, such as N2, Ar, He, etc., is called...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

DataAtlas: automatic generation of data dictionaries using large language models.

JAMIA open·2026
Same author

Integrated Downstream Analysis and Epidemiological Modelling of Hantavirus Infection: From Host Transcriptomics to Transmission Dynamics.

Pathogens (Basel, Switzerland)·2026
Same author

An Innovative 3D Slicer Plugin for Brain Images Annotation and Lesions Study.

Studies in health technology and informatics·2026
Same author

On the Ethical Aspect of Artificial Intelligence-Based Decision Process for Transplantation.

Studies in health technology and informatics·2026
Same author

An Automatic Data Extracting Method for REDCap Folder Mapping: An Example for Cardiological Clinical Case.

Studies in health technology and informatics·2026
Same author

Bidirectional Mamba-2 boosts EEG super-resolution via regression and diffusion.

Bioinformatics (Oxford, England)·2026
Same journal

Facial iPPG heatmap patterns based on period-aware autoencoder show association with carotid atherosclerosis towards non-contact hemodynamic assessment.

Computer methods and programs in biomedicine·2026
Same journal

Explainable machine learning models predict liver fibrosis risk and outcome in the general population: Development and multi-cohort external validation.

Computer methods and programs in biomedicine·2026
Same journal

Evaluation of surrogate endpoints for survival outcomes using the surrogate package in R.

Computer methods and programs in biomedicine·2026
Same journal

Relative spectral and frication-based descriptors as numerical indicators of place of articulation shifts in fricatives produced by Polish children.

Computer methods and programs in biomedicine·2026
Same journal

Leaflet resection improves valve expansion and hemodynamic performance in redo TAVI with balloon- and self-expanding transcatheter heart valve configurations.

Computer methods and programs in biomedicine·2026
Same journal

Spectral super-resolution for Parkinson's voice via representation-level methods under mixed-reality acquisition.

Computer methods and programs in biomedicine·2026
See all related articles

Related Experiment Video

Updated: Jun 24, 2026

Analyzing Large Protein Complexes by Structural Mass Spectrometry
15:35

Analyzing Large Protein Complexes by Structural Mass Spectrometry

Published on: June 19, 2010

MaSDA: a system for analyzing mass spectrometry data.

Francesco Gullo1, Giovanni Ponti, Andrea Tagarelli

  • 1Dept. of Electronics, Computer and Systems Sciences (DEIS), University of Calabria, Via P.Bucci 41c, Rende (CS) I87036, Italy. fgullo@deis.unical.it

Computer Methods and Programs in Biomedicine
|April 7, 2009
PubMed
Summary
This summary is machine-generated.

Mass spectrometry (MS) data analysis is challenging due to high dimensionality and noise. The MaSDA system offers advanced preprocessing and data mining tools for effective knowledge discovery from MS data.

More Related Videos

Navigating the Mass Spectrometry-Based Proteomic Data Using Free Computational Tools
07:01

Navigating the Mass Spectrometry-Based Proteomic Data Using Free Computational Tools

Published on: August 19, 2025

T-wave Ion Mobility-mass Spectrometry: Basic Experimental Procedures for Protein Complex Analysis
16:40

T-wave Ion Mobility-mass Spectrometry: Basic Experimental Procedures for Protein Complex Analysis

Published on: July 31, 2010

Related Experiment Videos

Last Updated: Jun 24, 2026

Analyzing Large Protein Complexes by Structural Mass Spectrometry
15:35

Analyzing Large Protein Complexes by Structural Mass Spectrometry

Published on: June 19, 2010

Navigating the Mass Spectrometry-Based Proteomic Data Using Free Computational Tools
07:01

Navigating the Mass Spectrometry-Based Proteomic Data Using Free Computational Tools

Published on: August 19, 2025

T-wave Ion Mobility-mass Spectrometry: Basic Experimental Procedures for Protein Complex Analysis
16:40

T-wave Ion Mobility-mass Spectrometry: Basic Experimental Procedures for Protein Complex Analysis

Published on: July 31, 2010

Area of Science:

  • Biomedical data analysis
  • Computational biology
  • Mass spectrometry applications

Background:

  • Mass spectrometry (MS) generates complex data requiring sophisticated analysis.
  • High dimensionality and noise in MS data pose significant challenges for clinical knowledge discovery.
  • Existing methods may not adequately address the complexities of MS data preprocessing and modeling.

Purpose of the Study:

  • To present MaSDA, a novel system for advanced analysis of mass spectrometry data.
  • To provide tools for effective handling, preprocessing, and knowledge discovery from MS data.
  • To assist clinicians in making critical decisions using MS data insights.

Main Methods:

  • Developed MaSDA, a system integrating MS data representation, preprocessing, and data mining.
  • Implemented a low-dimensional, dense time series model for MS data representation.
  • Integrated a user-friendly graphical tool for MS data preprocessing operations.
  • Embedded data mining and knowledge discovery tools for clinical decision support.

Main Results:

  • MaSDA effectively handles high-dimensional and noisy MS data.
  • The system demonstrates efficiency in preprocessing and advanced analysis of MS data.
  • Experimental testing on public datasets confirmed the system's effectiveness.

Conclusions:

  • MaSDA provides a robust platform for advanced mass spectrometry data analysis.
  • The system facilitates knowledge discovery and supports clinical decision-making.
  • MaSDA enhances the utility of MS data in biomedical research and clinical practice.