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Updated: Jun 24, 2026

Next-generation Sequencing of 16S Ribosomal RNA Gene Amplicons
Published on: August 29, 2014
Identification of ribosomal RNA genes in metagenomic fragments
Ying Huang1, Paul Gilna, Weizhong Li
1California Institute for Telecommunications and Information Technology, University of California, La Jolla, San Diego, California, USA.
Motivation:
Identification of genes coding for ribosomal RNA (rRNA) is considered an important goal in the analysis of data from metagenomics projects. Here, we report the development of a software program designed for the identification of rRNA genes from metagenomic fragments based on hidden Markov models (HMMs). This program provides rRNA gene predictions with high sensitivity and specificity on artificially fragmented genomic DNAs.
Availability:
Supplementary files, scripts and sample data are available at http://tools.camera.calit2.net/camera/meta_rna.
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