Related Experiment Video

Updated: Jun 24, 2026

Rapid Isolation of the Mitoribosome from HEK Cells
09:33

Rapid Isolation of the Mitoribosome from HEK Cells

Published on: October 4, 2018

The amino terminal domain from Mrt4 protein can functionally replace the RNA binding domain of the ribosomal P0

María Rodríguez-Mateos1, David Abia, Juan J García-Gómez

  • 1Centro de Biología Molecular Severo Ochoa, Consejo Superior de Investigaciones Científicas and Universidad Autónoma de Madrid, Cantoblanco, Madrid 28049, Spain.

Nucleic Acids Research
|April 7, 2009
PubMed

Insights

Mrt4 and P0 proteins in Saccharomyces cerevisiae cannot bind ribosomes simultaneously. A chimera protein partially rescues P0 absence, indicating Mrt4 aids P0 ribosome assembly.

Area of Science:

  • Molecular Biology
  • Yeast Genetics
  • Ribosome Biogenesis

Background:

  • Mrt4 protein in Saccharomyces cerevisiae is involved in ribosome assembly.
  • Mrt4 shares sequence homology with the P0 ribosomal stalk protein.
  • The precise role of Mrt4 in ribosome assembly remains unclear.

Purpose of the Study:

  • To investigate the functional relationship between Mrt4 and P0 proteins.
  • To determine if Mrt4 and P0 compete for the same binding site on the ribosome.
  • To elucidate Mrt4's role in the assembly of ribosomal stalk proteins.

Main Methods:

  • Construction and testing of an Mrt4-P0 chimera protein in a P0 null mutant.
  • Analysis of ribosome composition and protein binding in yeast strains.
  • Molecular dynamics simulations of Mrt4 and P0 interactions with rRNA and L12.

Main Results:

  • Mrt4 and P0 cannot bind simultaneously to ribosomes.
  • The Mrt4-P0 chimera partially complements the P0 null mutation.
  • Chimera binding to ribosomes is weaker than P0 and affects P1, P2, and L12 protein association.
  • Simulations suggest Mrt4 and P0 bind to the same rRNA site but interact differently with L12.

Conclusions:

  • Mrt4 and P0 compete for the same binding site on 25S rRNA.
  • Mrt4 likely participates in the ribosome assembly of P0 and potentially L12 proteins.
  • These findings clarify Mrt4's role in the structural organization of the ribosomal stalk.

More Related Videos

Novel RNA-Binding Proteins Isolation by the RaPID Methodology11:19

Novel RNA-Binding Proteins Isolation by the RaPID Methodology

Published on: September 30, 2016

Isolation of Translating Ribosomes Containing Peptidyl-tRNAs for Functional and Structural Analyses11:19

Isolation of Translating Ribosomes Containing Peptidyl-tRNAs for Functional and Structural Analyses

Published on: February 25, 2011

Related Concept Videos

Nucleic Acid Structure01:25

Nucleic Acid Structure

The pentose sugar in DNA is deoxyribose, while in RNA the pentose sugar is ribose. The difference between the sugars is the presence of the hydroxyl group on the ribose's second carbon and a hydrogen on the deoxyribose's second carbon. The phosphate residue attaches to the hydroxyl group of the 5′ carbon of one sugar and the hydroxyl group of the 3′ carbon of the sugar of the next nucleotide, which forms  a 5′ to 3′ phosphodiester linkage.
DNA Structure
DNA has a double-helix structure. The...
Nucleic Acids02:43

Nucleic Acids

Nucleic acids are the most important macromolecules for the continuity of life. They carry the cell's genetic blueprint and carry instructions for its functioning.
DNA and RNA
The two main types of nucleic acids are deoxyribonucleic acid (DNA) and ribonucleic acid (RNA). DNA is the genetic material in all living organisms, ranging from single-celled bacteria to multicellular mammals. It is in the nucleus of eukaryotes and in the organelles, chloroplasts, and mitochondria. In prokaryotes, the...
Nucleic acids02:43

Nucleic acids

Nucleic acids are the most important macromolecules for the continuity of life. They carry the cell's genetic blueprint and carry instructions for its functioning.
DNA and RNA
The two main types of nucleic acids are deoxyribonucleic acid (DNA) and ribonucleic acid (RNA). DNA is the genetic material in all living organisms, ranging from single-celled bacteria to multicellular mammals. It is in the nucleus of eukaryotes and in the organelles, chloroplasts, and mitochondria. In prokaryotes, the...
Ribosomal RNA Synthesis02:53

Ribosomal RNA Synthesis

Ribosome synthesis is a highly complex and coordinated process involving more than 200 assembly factors. The synthesis and processing of ribosomal components occurs not only in the nucleolus but also in the nucleoplasm and the cytoplasm of eukaryotic cells.
Ribosome biogenesis begins with the synthesis of 5S and 45S pre-rRNAs by distinct RNA polymerases. The primary transcripts are extensively processed and modified before they are bound and folded by ribosomal proteins and assembly factors,...
Ribosomal RNA Synthesis02:53

Ribosomal RNA Synthesis

Ribosome synthesis is a highly complex and coordinated process involving more than 200 assembly factors. The synthesis and processing of ribosomal components occurs not only in the nucleolus but also in the nucleoplasm and the cytoplasm of eukaryotic cells.
Ribosome biogenesis begins with the synthesis of 5S and 45S pre-rRNAs by distinct RNA polymerases. The primary transcripts are extensively processed and modified before they are bound and folded by ribosomal proteins and assembly factors,...
RNA Structure01:19

RNA Structure

The basic structure of RNA consists of a string of ribonucleotides attached by phosphodiester bonds. Although most RNA is single-stranded, it can form complex secondary and tertiary structures. Such structures play essential roles in the regulation of transcription and translation.
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA) involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three...