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Her-2/neu, p-53, and their coexpression in osteosarcoma
Sameer Bakhshi1, Ajay Gupta, Meher C Sharma
1Department of Medical Oncology, Institute Rotary Cancer Hospital, All India Institute of Medical Sciences, New Delhi, India. sambakh@hotmail.com
This study investigated Her-2/neu and p-53 expression in osteosarcoma, finding no direct link to tumor grade or stage. However, chondroblastic subtype correlated with Her-2/neu staining, and coexpression linked to membranous Her-2/neu.
Area of Science:
- Oncology
- Molecular Pathology
- Cancer Biomarkers
Background:
- Controversy exists regarding the prognostic significance of Her-2/neu and p-53 expression in osteosarcoma.
- The combined expression patterns of Her-2/neu and p-53 in osteosarcoma have not been previously investigated.
Purpose of the Study:
- To analyze the expression patterns of Her-2/neu and p-53 in osteosarcoma.
- To evaluate the association of these expressions with tumor grade, stage, and morphologic subtypes.
- To investigate the prognostic significance of their combined expression.
Main Methods:
- Immunohistochemistry was used to analyze Her-2/neu and p-53 expression in prechemotherapy biopsy specimens from 63 osteosarcoma patients.
- Tumor characteristics including grade, stage, and morphologic subtype were compared with protein expression levels.
Main Results:
- Her-2/neu expression was observed in 47.1% of cases, with 3+ staining grade associated with the chondroblastic subtype (P=0.026).
- p-53 expression was detected in 31.74% of cases, showing higher prevalence in chondroblastic and fibroblastic subtypes.
- Coexpression of Her-2/neu and p-53 occurred in 15.87% of cases and was significantly associated with membranous Her-2/neu staining (P=0.01).
Conclusions:
- Her-2/neu and p-53 expression, individually or combined, were not found to be related to tumor grade or stage in osteosarcoma.
- Chondroblastic osteosarcoma subtype showed a significant association with a high staining grade of Her-2/neu.
- The coexpression of Her-2/neu and p-53 was significantly linked to membranous Her-2/neu staining patterns.
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