Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Glucose Transporters01:27

Glucose Transporters

Glucose transporters facilitate the transport of glucose across the cell membrane. In addition to glucose, some glucose transporters can also aid the movement of other hexoses such as fructose, mannose, and galactose.
Facilitated diffusion-glucose transporters (GLUTs) are encoded by the solute-linked carrier (SLC) family 2, subfamily A gene family, or SLC2A. The 14 GLUT protein members are distributed into three classes:
Membrane Proteins01:30

Membrane Proteins

Plasma membranes have integral transmembrane proteins involved in facilitated transport. These proteins are collectively referred to as transport proteins, and they function as either channels for the material or as carriers themselves. Channel proteins have hydrophilic domains exposed to the intracellular and extracellular fluids and a hydrophilic channel through their core that provides a hydrated opening for solutes to pass through the membrane layers. Passage through the channel allows...
Secondary Active Transport01:32

Secondary Active Transport

One example of how cells use the energy contained in electrochemical gradients is demonstrated by glucose transport into cells. The ion vital to this process is sodium (Na+), which is typically present in higher concentrations extracellularly than in the cytosol. Such a concentration difference is due, in part, to the action of an enzyme "pump" embedded in the cellular membrane that actively expels Na+ from a cell. Importantly, as this pump contributes to the high concentration of...
Secondary Active Transport01:55

Secondary Active Transport

One example of how cells use the energy contained in electrochemical gradients is demonstrated by glucose transport into cells. The ion vital to this process is sodium (Na+), which is typically present in higher concentrations extracellularly than in the cytosol. Such a concentration difference is due, in part, to the action of an enzyme “pump” embedded in the cellular membrane that actively expels Na+ from a cell. Importantly, as this pump contributes to the high concentration of...
Secondary Active Transport01:32

Secondary Active Transport

One example of how cells use the energy contained in electrochemical gradients is demonstrated by glucose transport into cells. The ion vital to this process is sodium (Na+), which is typically present in higher concentrations extracellularly than in the cytosol. Such a concentration difference is due, in part, to the action of an enzyme "pump" embedded in the cellular membrane that actively expels Na+ from a cell. Importantly, as this pump contributes to the high concentration of...
Glucose Absorption Into the Small Intestine01:26

Glucose Absorption Into the Small Intestine

Complex carbohydrates consumed cannot be absorbed into the small intestine in their original form. First, they must be hydrolyzed to a monosaccharide form such as glucose or galactose. These monosaccharides are then transported across the intestinal membrane and into the blood via transcellular transport. The intestinal epithelial cells allow the movement of these monosaccharides with a defined 'entry' through membrane transporter proteins present on their apical membrane and 'exit' via the...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

ZEB1 drives terminal erythroid maturation by controlling the GATA2-KLF1 regulatory switch.

Nucleic acids research·2026
Same author

In Remembrance of Professor Zelig Eshhar: <i>A Life Committed to CAR-T</i>.

Human gene therapy·2026
Same author

CD19/CD22 bivalent CAR T cells in children, adolescents and young adults with B-ALL: final phase 1 trial results.

Journal for immunotherapy of cancer·2026
Same author

Baricitinib for refractory chronic graft-versus-host-disease: Results of a phase 1/2 study.

Blood advances·2026
Same author

Single-cell lineage tracing identifies hemogenic endothelial cells in the adult mouse bone marrow.

eLife·2026
Same author

Evaluation of common urine leukocyte dipsticks for diagnosing peritoneal dialysis (PD)-associated peritonitis.

Peritoneal dialysis international : journal of the International Society for Peritoneal Dialysis·2026

Related Experiment Video

Updated: Jun 24, 2026

Extracellular Glucose Depletion as an Indirect Measure of Glucose Uptake in Cells and Tissues Ex Vivo
10:35

Extracellular Glucose Depletion as an Indirect Measure of Glucose Uptake in Cells and Tissues Ex Vivo

Published on: April 6, 2022

Erythroid glucose transporters.

Amélie Montel-Hagen1, Marc Sitbon, Naomi Taylor

  • 1Institut de Génétique Moléculaire de Montpellier, CNRS UMR 5535/IFR122, Université Montpellier I and II, Montpellier Cedex 5, France.

Current Opinion in Hematology
|April 7, 2009
PubMed
Summary
This summary is machine-generated.

Mammalian red blood cells (erythrocytes) show varied expression of glucose transporters like GLUT1. This variation impacts vitamin C transport and glucose uptake, revealing new insights into red cell metabolism.

More Related Videos

Measuring Uptake of the Glucose Analog, 6-(N-(7-Nitrobenz-2-Oxa-1,3-Diazol-4-yl)Amino)-6-Deoxyglucose, in Intact Murine Neural Retina
07:04

Measuring Uptake of the Glucose Analog, 6-(N-(7-Nitrobenz-2-Oxa-1,3-Diazol-4-yl)Amino)-6-Deoxyglucose, in Intact Murine Neural Retina

Published on: March 14, 2025

Glucose Uptake Measurement and Response to Insulin Stimulation in In Vitro Cultured Human Primary Myotubes
08:03

Glucose Uptake Measurement and Response to Insulin Stimulation in In Vitro Cultured Human Primary Myotubes

Published on: June 25, 2017

Related Experiment Videos

Last Updated: Jun 24, 2026

Extracellular Glucose Depletion as an Indirect Measure of Glucose Uptake in Cells and Tissues Ex Vivo
10:35

Extracellular Glucose Depletion as an Indirect Measure of Glucose Uptake in Cells and Tissues Ex Vivo

Published on: April 6, 2022

Measuring Uptake of the Glucose Analog, 6-(N-(7-Nitrobenz-2-Oxa-1,3-Diazol-4-yl)Amino)-6-Deoxyglucose, in Intact Murine Neural Retina
07:04

Measuring Uptake of the Glucose Analog, 6-(N-(7-Nitrobenz-2-Oxa-1,3-Diazol-4-yl)Amino)-6-Deoxyglucose, in Intact Murine Neural Retina

Published on: March 14, 2025

Glucose Uptake Measurement and Response to Insulin Stimulation in In Vitro Cultured Human Primary Myotubes
08:03

Glucose Uptake Measurement and Response to Insulin Stimulation in In Vitro Cultured Human Primary Myotubes

Published on: June 25, 2017

Area of Science:

  • Cell Biology
  • Biochemistry
  • Comparative Physiology

Background:

  • Animals are heterotrophic, utilizing sugars for energy.
  • All cells possess hexose transporters; human erythrocytes (red blood cells) exhibit high levels of facilitative glucose transporter 1 (GLUT1).
  • Previous assumptions suggested universal GLUT1 expression and similar function across mammalian erythrocytes.

Purpose of the Study:

  • To investigate the species-specific expression and function of glucose transporters in mammalian erythrocytes.
  • To re-evaluate the assumption of conserved GLUT1 function in red blood cells across different mammalian species.

Main Methods:

  • Comparative analysis of erythrocyte membrane proteins and glucose transport activity across diverse mammalian species.
  • Investigated the role of GLUT1 and its association with stomatin in L-dehydroascorbic acid uptake.
  • Examined the expression patterns of GLUT4 in specific species like mice.

Main Results:

  • GLUT1 expression in erythrocytes is not universal, found mainly in higher primates, guinea pigs, and fruit bats, which cannot synthesize ascorbic acid.
  • Human erythrocytes show increased L-dehydroascorbic acid uptake via GLUT1, regulated by stomatin.
  • Ascorbic acid-producing species have limited erythroid GLUT1 expression to fetal/neonatal stages; murine erythrocytes utilize GLUT4 for glucose transport later.

Conclusions:

  • Erythrocyte expression of GLUT-type transporters differs significantly among mammalian species.
  • The function of these transporters in red blood cells can vary, leading to novel understandings of red cell metabolism and nutrient transport.