Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Bone histogenesis mediated by nonosteogenic cells.

K H Włodarski1

  • 1Department of Histology and Embryology, Medical Academy, Warszawa, Poland.

Clinical Orthopaedics and Related Research
|November 1, 1991
PubMed
Summary

Epithelial cells implanted into muscle can induce new bone formation and bone marrow development. This ectopic bone formation is similar to natural bone marrow and may involve factors produced during anti-tumor responses.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Tumor induced by Moloney sarcoma virus causes periosteal osteogenesis engaging osteopontin, fibronectin, stromelysin-1 and tenascin.

Neoplasma·2007
Same author

Fluvastatin increases heterotopically induced ossicles in mice.

Clinical and experimental pharmacology & physiology·2006
Same author

Non-adherent bone marrow cells are a rich source of cells forming bone in vivo.

Folia biologica·2004
Same author

Evaluation of locally induced osteoarthritis by the complete and incomplete Freund's adjuvant in mice. The application of DEXA measurements.

Folia biologica·2002
Same author

Peculiarities of the rodent subcutaneous space for testing of bone inducing agents.

Growth factors (Chur, Switzerland)·2001
Same author

Does colchicine really induce bone formation in the rodent bone marrow?

Calcified tissue international·1997

Area of Science:

  • Biomedical Science
  • Cell Biology
  • Tissue Engineering

Background:

  • Epithelial cells, when implanted into muscle, can induce endochondral ossification.
  • Heterotopically induced bone supports myelopoiesis, with bone marrow characteristics similar to orthotopic bone marrow.
  • Ectopic bone lacks a periosteal membrane with a functional cambium layer.

Purpose of the Study:

  • To investigate the process of heterotopic bone formation induced by epithelial cell grafts.
  • To characterize the nature of the bone-inducing principle and the responding cells.
  • To explore the potential role of anti-tumor reaction factors in bone formation.

Main Methods:

  • Intramuscular implantation of established epithelial cell lines.
  • Analysis of induced bone tissue, including myelopoiesis and histological examination.

Related Experiment Videos

  • Comparison of ectopic bone marrow with orthotopic bone marrow.
  • Investigation of periosteal and perichondral membrane activation by various agents.

    • Main Results:

    • Epithelial cell grafts induced endochondral ossification and myelopoiesis in muscle tissue.
    • Ectopic bone marrow composition was comparable to normal bone marrow.
    • Bone-inducing epithelial cells exhibited high alkaline phosphatase activity and epithelial status.
    • Periosteal and perichondral membranes were activated by various agents, suggesting a role for cellular factors.

    Conclusions:

    • Epithelial cells possess the capacity to induce ectopic bone formation and functional bone marrow.
    • The bone-inducing principle is linked to epithelial cell characteristics, including high alkaline phosphatase activity.
    • Factors involved in anti-tumor immune responses may contribute to chondroblast/osteoblast activation and bone formation.