Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

SyntheMol-RL: a flexible reinforcement learning framework for designing easily synthesizable antibiotics.

Molecular systems biology·2026
Same author

Butyrolactol A enhances caspofungin efficacy via flippase inhibition in drug-resistant fungi.

Cell·2026
Same author

Evaluation of Azithromycin-Bicarbonate against Multidrug-Resistant Pathogens in Topical Murine Models of Infection.

ACS infectious diseases·2025
Same author

A conserved sRNA regulates mucin adhesion and gut colonization across the Enterococcaceae.

bioRxiv : the preprint server for biology·2025
Same author

Author Correction: Discovery and artificial intelligence-guided mechanistic elucidation of a narrow-spectrum antibiotic.

Nature microbiology·2025
Same author

Discovery and artificial intelligence-guided mechanistic elucidation of a narrow-spectrum antibiotic.

Nature microbiology·2025

Related Experiment Video

Updated: Jun 24, 2026

High Throughput, Real-time, Dual-readout Testing of Intracellular Antimicrobial Activity and Eukaryotic Cell Cytotoxicity
09:09

High Throughput, Real-time, Dual-readout Testing of Intracellular Antimicrobial Activity and Eukaryotic Cell Cytotoxicity

Published on: November 16, 2016

High-throughput screening of model bacteria.

Soumaya Zlitni1, Jan E Blanchard, Eric D Brown

  • 1Department of Biochemistry and Biomedical Sciences, McMaster University, Hamilton, ON, Canada.

Methods in Molecular Biology (Clifton, N.J.)
|April 7, 2009
PubMed
Summary

Academic researchers can now screen for novel antibacterial compounds using high-throughput methods. This approach identifies small molecules that inhibit bacterial growth, aiding biological research.

More Related Videos

Establishment of a High-throughput Setup for Screening Small Molecules That Modulate c-di-GMP Signaling in Pseudomonas aeruginosa
11:31

Establishment of a High-throughput Setup for Screening Small Molecules That Modulate c-di-GMP Signaling in Pseudomonas aeruginosa

Published on: June 30, 2016

A High Throughput Screen for Biomining Cellulase Activity from Metagenomic Libraries
10:21

A High Throughput Screen for Biomining Cellulase Activity from Metagenomic Libraries

Published on: February 1, 2011

Related Experiment Videos

Last Updated: Jun 24, 2026

High Throughput, Real-time, Dual-readout Testing of Intracellular Antimicrobial Activity and Eukaryotic Cell Cytotoxicity
09:09

High Throughput, Real-time, Dual-readout Testing of Intracellular Antimicrobial Activity and Eukaryotic Cell Cytotoxicity

Published on: November 16, 2016

Establishment of a High-throughput Setup for Screening Small Molecules That Modulate c-di-GMP Signaling in Pseudomonas aeruginosa
11:31

Establishment of a High-throughput Setup for Screening Small Molecules That Modulate c-di-GMP Signaling in Pseudomonas aeruginosa

Published on: June 30, 2016

A High Throughput Screen for Biomining Cellulase Activity from Metagenomic Libraries
10:21

A High Throughput Screen for Biomining Cellulase Activity from Metagenomic Libraries

Published on: February 1, 2011

Area of Science:

  • Microbiology and Drug Discovery
  • Bacterial Physiology and Biochemistry

Background:

  • Small-molecule screening is crucial for discovering novel antibacterial compounds.
  • High-throughput screening (HTS) is increasingly adopted in academic research for biological studies.
  • Whole-cell screening offers advantages in identifying cell-permeable compounds.

Purpose of the Study:

  • To describe a general method for conducting high-throughput screening against model bacteria.
  • To identify small molecules exhibiting growth inhibitory activity.
  • To characterize the dose-response relationships of identified bioactive compounds.

Main Methods:

  • Implementation of a high-throughput screening (HTS) campaign.
  • Utilizing whole-cell screening to assess compound efficacy.
  • Determining dose-response relationships for primary bioactive compounds.

Main Results:

  • Identification of small molecules with growth inhibitory activity against a model bacterium.
  • Characterization of the inhibitory effects through dose-response analysis.

Conclusions:

  • The described HTS method provides a robust approach for academic labs to discover antibacterial agents.
  • Whole-cell screening is effective for identifying compounds with potential for microbial cell permeation.
  • This methodology facilitates the study of microbial physiology and biochemistry using novel chemical probes.