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Reporter Genes

Reporter Genes

Reporter genes are a type of protein-coding gene that are often tagged to a gene of interest. Once inside a target cell, reporter genes usually produce visually identifiable characteristics like fluorescence and luminescence when expressed along with the gene of interest. Thus, reporter genes “report” the presence or absence of genes of interest in an organism, determine the gene expression pattern, or track the physical location of a DNA segment or protein in the cell.
Commonly used reporter...

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Related Experiment Video

Updated: Jun 24, 2026

Massively Parallel Reporter Assays in Cultured Mammalian Cells

Massively Parallel Reporter Assays in Cultured Mammalian Cells

Published on: August 17, 2014

Reporter gene assays.

Andy M F Liu¹, David C New, Rico K H Lo

¹Department of Biochemistry, Molecular Neuroscience Center, Biotechnology Research Institute, Hong Kong University of Science and Technology, Hong Kong, China.

Methods in Molecular Biology (Clifton, N.J.)

|April 7, 2009

Summary

This summary is machine-generated.

Reporter gene assays offer sensitive, versatile screening for drug discovery. This work details luciferase and aequorin assays for G protein-coupled receptor ligand screening.

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Area of Science:

Biochemistry
Molecular Biology
Pharmacology

Background:

Reporter gene assays are crucial for high-throughput drug screening.
Diverse reporter genes and promoters allow tailored assay development.
Assay selection depends on sensitivity, detection, cellular system, and multiplexing needs.

Purpose of the Study:

To guide the selection of appropriate reporter gene assays.
To describe the establishment of 96-well format luciferase and aequorin assays.
To facilitate the screening of ligands targeting G protein-coupled receptors.

Main Methods:

Utilized reporter gene assays for target validation.
Developed 96-well format assays using luciferase and aequorin reporters.
Employed promoters responsive to various biochemical pathways.

Main Results:

Established sensitive and versatile reporter gene assays.
Successfully adapted assays for screening G protein-coupled receptor ligands.
Demonstrated compatibility with high-throughput screening.

Conclusions:

Reporter gene assays are adaptable tools for drug discovery.
Luciferase and aequorin assays provide robust methods for GPCR ligand screening.
Assay optimization is key for successful high-throughput screening programs.