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Related Experiment Video

Updated: Jun 24, 2026

Efficient Polyethylene Glycol (PEG) Mediated Transformation of the Moss Physcomitrella patens

Efficient Polyethylene Glycol (PEG) Mediated Transformation of the Moss Physcomitrella patens

Published on: April 19, 2011

Stable transformation of plants.

Huw D Jones¹, Caroline A Sparks

¹CPI Division, Rothamsted Research, Harpenden, Hertfordshire, UK.

Methods in Molecular Biology (Clifton, N.J.)

|April 7, 2009

Summary

This study outlines key steps for producing stably transformed plants using tissue culture. It details explant preparation, deoxyribonucleic acid (DNA) delivery via particle bombardment, and regeneration for wheat.

Area of Science:

Plant biotechnology
Molecular biology
Agricultural science

Background:

Plant transformation is crucial for crop improvement.
Tissue culture techniques are essential for regenerating whole plants from modified cells.
Efficient deoxyribonucleic acid (DNA) delivery is a key challenge in plant biotechnology.

Purpose of the Study:

To provide a comprehensive overview of plant transformation protocols.
To detail the stages involved in producing stably transformed plants.
To present a specific protocol for wheat transformation using particle bombardment.

Main Methods:

The study outlines a three-stage process: explant preparation, DNA delivery, and regeneration/selection.
Focuses on particle bombardment for deoxyribonucleic acid (DNA) delivery.

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Agrobacterium tumefaciens-Mediated Genetic Transformation of Narrowleaf Plantain

Agrobacterium tumefaciens-Mediated Genetic Transformation of Narrowleaf Plantain

Published on: March 17, 2023

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Efficient Polyethylene Glycol (PEG) Mediated Transformation of the Moss Physcomitrella patens

Efficient Polyethylene Glycol (PEG) Mediated Transformation of the Moss Physcomitrella patens

Published on: April 19, 2011

Agrobacterium tumefaciens-Mediated Genetic Transformation of Narrowleaf Plantain

Agrobacterium tumefaciens-Mediated Genetic Transformation of Narrowleaf Plantain

Published on: March 17, 2023

Utilizes tissue culture for plant regeneration from explants.

Main Results:

A detailed protocol for producing stably transformed wheat is presented.
Particle bombardment is highlighted as an effective method for deoxyribonucleic acid (DNA) delivery in wheat.
The general principles of plant transformation are illustrated through the wheat exemplar.

Conclusions:

The described methodology provides a framework for generating stably transformed plants.
Particle bombardment offers a viable alternative for deoxyribonucleic acid (DNA) delivery in species recalcitrant to other methods.
Successful plant transformation relies on careful execution of explant preparation, gene delivery, and regeneration stages.