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Related Experiment Video

Updated: Jun 24, 2026

Generating Transgenic Plants with Single-copy Insertions Using BIBAC-GW Binary Vector
12:08

Generating Transgenic Plants with Single-copy Insertions Using BIBAC-GW Binary Vector

Published on: March 28, 2018

A simple TAE-based method to generate large insert BAC libraries from plant species.

Bu-Jun Shi1, J Perry Gustafson, Peter Langridge

  • 1Australian Centre for Plant Functional Genomics, University of Adelaide, Glen Osmond, SA 5064, Australia.

Methods in Molecular Biology (Clifton, N.J.)
|April 7, 2009
PubMed
Summary

This study presents a simplified method for constructing bacterial artificial chromosome (BAC) libraries, crucial for plant genomics and breeding. The efficient protocol aids in gene cloning and physical mapping across diverse plant species.

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Area of Science:

  • Genomics
  • Molecular Biology
  • Plant Science

Background:

  • Large DNA insert libraries are essential for gene cloning, physical mapping, comparative genomics, and molecular breeding.
  • Bacterial artificial chromosome (BAC) libraries are widely utilized due to their large insert capacity, stability, and ease of manipulation.

Purpose of the Study:

  • To report a simplified and efficient method for constructing plant BAC libraries.
  • To facilitate the generation of BAC libraries for various plant species.

Main Methods:

  • Isolation and partial digestion of intact plant nuclei.
  • Pulsed-field gel electrophoresis (PFG) for DNA size selection.
  • Ligation of DNA into BAC vectors and electroporation into Escherichia coli.

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Robust DNA Isolation and High-throughput Sequencing Library Construction for Herbarium Specimens
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Robust DNA Isolation and High-throughput Sequencing Library Construction for Herbarium Specimens

Published on: March 8, 2018

Related Experiment Videos

Last Updated: Jun 24, 2026

Generating Transgenic Plants with Single-copy Insertions Using BIBAC-GW Binary Vector
12:08

Generating Transgenic Plants with Single-copy Insertions Using BIBAC-GW Binary Vector

Published on: March 28, 2018

Generating Transposon Insertion Libraries in Gram-Negative Bacteria for High-Throughput Sequencing
08:19

Generating Transposon Insertion Libraries in Gram-Negative Bacteria for High-Throughput Sequencing

Published on: July 7, 2020

Robust DNA Isolation and High-throughput Sequencing Library Construction for Herbarium Specimens
13:03

Robust DNA Isolation and High-throughput Sequencing Library Construction for Herbarium Specimens

Published on: March 8, 2018

Main Results:

  • A simplified protocol for plant BAC library construction is detailed.
  • The method has been successfully applied to create BAC libraries from sunolgrass, barley, lupin, and rye.
  • The entire process is estimated to take 1-3 months, depending on genome size and desired coverage.

Conclusions:

  • The described method offers a streamlined approach to plant BAC library construction.
  • This technique supports advancements in plant genomics, molecular breeding, and genetic research.