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Published on: August 27, 2021
P087, a lactococcal phage with a morphogenesis module similar to an Enterococcus faecalis prophage
Manuela Villion1, Marie-Christine Chopin, Hélène Deveau
1Département de Biochimie et de Microbiologie, Faculté des Sciences et de Génie, Université Laval, Québec, Québec, Canada.
The virulent lactococcal phage P087, a reference member for Lactococcus lactis infections, has a unique genome structure. Analysis suggests potential DNA exchange with Enterococcus faecalis prophages in similar environments.
Area of Science:
- Microbiology
- Virology
- Genomics
Background:
- The virulent lactococcal phage P087 is a key member of a known phage group infecting Lactococcus lactis.
- Phage P087 was isolated from a dairy environment in 1978.
Purpose of the Study:
- To characterize the genome and structural proteins of lactococcal phage P087.
- To investigate potential genomic similarities and DNA exchange between phage P087 and other bacterial phages.
Main Methods:
- Genome sequencing and analysis of phage P087.
- Identification of structural proteins using SDS-PAGE and LC-MS/MS.
- Comparative genomic analysis with Enterococcus faecalis prophage sequences.
Main Results:
- Phage P087 possesses a 60,074 bp double-stranded DNA genome with 5 tRNAs and 88 ORFs, exhibiting an uncommon architecture.
- Eleven structural proteins were identified.
- Eleven translated ORFs from phage P087 showed homology to gene products of an Enterococcus faecalis V583 prophage.
Conclusions:
- The genome architecture of phage P087 is unusual.
- Genomic sequence alignment suggests potential DNA exchange between phage P087 and Enterococcus faecalis prophages.
- These phages infect low G+C bacteria residing in similar ecological niches, supporting the possibility of horizontal gene transfer.
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