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Related Concept Videos

RNA Splicing01:32

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
RNA Splicing01:32

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
Pre-mRNA Processing: RNA Splicing01:32

Pre-mRNA Processing: RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
Protein Complexes with Interchangeable Parts01:57

Protein Complexes with Interchangeable Parts

Groups of proteins may form a complex where each protein in this complex has a different role in the overall execution of the complex’s function. Often some of the proteins in the complex can be replaced by a closely related variant to give a complex that contains many of the same components yet is functionally distinct.
The SCF ubiquitin ligase is a protein complex of five individual proteins. This complex attaches ubiquitin to other target proteins to mark them for degradation. In order to...
Protein Complexes with Interchangeable Parts01:57

Protein Complexes with Interchangeable Parts

Groups of proteins may form a complex where each protein in this complex has a different role in the overall execution of the complex’s function. Often some of the proteins in the complex can be replaced by a closely related variant to give a complex that contains many of the same components yet is functionally distinct.
The SCF ubiquitin ligase is a protein complex of five individual proteins. This complex attaches ubiquitin to other target proteins to mark them for degradation. In order to...
Alternative RNA Splicing02:18

Alternative RNA Splicing

Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...

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Related Experiment Video

Updated: Jun 24, 2026

ACT1-CUP1 Assays Determine the Substrate-Specific Sensitivities of Spliceosomal Mutants in Budding Yeast
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ACT1-CUP1 Assays Determine the Substrate-Specific Sensitivities of Spliceosomal Mutants in Budding Yeast

Published on: June 30, 2022

Functional association of the Microprocessor complex with the spliceosome.

Naoyuki Kataoka1, Megumi Fujita, Mutsuhito Ohno

  • 1Institute for Virus Research, Kyoto University, Kyoto 606-8507, Japan. kataoka.mtt@mri.tmd.ac.jp

Molecular and Cellular Biology
|April 8, 2009
PubMed
Summary
This summary is machine-generated.

Most human microRNAs (miRNAs) originate within introns. A new in vitro system reveals Drosha-mediated miRNA cropping can precede spliceosome-mediated splicing and trans-splicing of intronic sequences.

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A Reporter Based Cellular Assay for Monitoring Splicing Efficiency
08:53

A Reporter Based Cellular Assay for Monitoring Splicing Efficiency

Published on: September 15, 2021

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Last Updated: Jun 24, 2026

ACT1-CUP1 Assays Determine the Substrate-Specific Sensitivities of Spliceosomal Mutants in Budding Yeast
07:31

ACT1-CUP1 Assays Determine the Substrate-Specific Sensitivities of Spliceosomal Mutants in Budding Yeast

Published on: June 30, 2022

A Reporter Based Cellular Assay for Monitoring Splicing Efficiency
08:53

A Reporter Based Cellular Assay for Monitoring Splicing Efficiency

Published on: September 15, 2021

Area of Science:

  • Molecular Biology
  • Genetics
  • RNA Biology

Background:

  • The majority of human microRNAs (miRNAs) are encoded within introns of protein-coding genes.
  • A previous model proposed that Drosha processes pre-miRNAs from introns, followed by trans-splicing of the remaining intronic fragments.
  • The molecular mechanisms underlying this intronic miRNA processing pathway remained unelucidated.

Purpose of the Study:

  • To investigate the molecular mechanism of intronic microRNA (miRNA) processing.
  • To elucidate the relationship between pre-miRNA processing and messenger RNA (mRNA) splicing.
  • To provide molecular evidence for a pathway involving Drosha, the spliceosome, and trans-splicing of intronic miRNAs.

Main Methods:

  • Development of an in vitro system to simultaneously detect pre-miRNA processing and mRNA splicing.
  • Glycerol gradient sedimentation to assess co-fractionation of pre-miRNA with the spliceosome.
  • Co-immunoprecipitation with an anti-Drosha antibody to identify associated splicing intermediates.

Main Results:

  • Pre-miRNA cropping from pre-mRNA was found to occur kinetically faster than splicing.
  • A portion of pre-miRNA co-fractionated with the spliceosome.
  • Drosha was shown to associate with pre-miRNA cropping products, a Y-shaped branch intron, and a Y-shaped splicing intermediate.

Conclusions:

  • The Microprocessor complex (including Drosha) associates with the spliceosome.
  • Pre-miRNA cropping precedes spliceosome-mediated splicing and subsequent trans-splicing of intronic fragments.
  • These findings provide a molecular basis for a novel pathway of intronic miRNA biogenesis.