Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Modern Molecular Taxonomy

Modern Molecular Taxonomy

Advancements in molecular biology have revolutionized the identification and characterization of bacteria, with multiple methods leveraging DNA sequencing for enhanced precision. As sequencing technologies improve and costs decline, these approaches are increasingly used in clinical, environmental, and evolutionary studies.Multilocus Sequence Typing (MLST) examines several housekeeping genes, essential chromosomal genes encoding cellular functions, to distinguish strains. Approximately...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Pyramiding of genes for grain protein content, grain quality, and rust resistance in eleven Indian bread wheat cultivars: a multi-institutional effort.

Molecular breeding : new strategies in plant improvement·2023

Same author

Understanding evolution in Poales: Insights from Eriocaulaceae plastome.

PloS one·2019

Same author

Validation and identification of molecular markers linked to the leaf rust resistance gene Lr28 in wheat.

Journal of applied genetics·2011

See all related articles

Search research articles

Related Experiment Video

Updated: May 1, 2026

Chromatin Interaction Analysis with Paired-End Tag Sequencing ChIA-PET for Mapping Chromatin Interactions and Understanding Transcription Regulation

Chromatin Interaction Analysis with Paired-End Tag Sequencing ChIA-PET for Mapping Chromatin Interactions and Understanding Transcription Regulation

Published on: April 30, 2012

Molecular profiling of "Chirayat" complex using Inter Simple Sequence Repeat (ISSR) markers.

Shubhada Tamhankar¹, Vinaya Ghate, Ajit Raut

¹Plant Sciences Division, Agharkar Research Institute, Pune, India.

|April 8, 2009

Summary

Inter-Simple Sequence Repeat (ISSR) markers effectively identified 12 "Chirayat" taxa, including Swertia species. This molecular profiling ensures the accurate authentication of medicinal plants and crude drug samples.

More Related Videos

Identifying Mutations by High Resolution Melting in a TILLING Population of Rice

Identifying Mutations by High Resolution Melting in a TILLING Population of Rice

Published on: September 2, 2019

A Robust Polymerase Chain Reaction-based Assay for Quantifying Cytosine-guanine-guanine Trinucleotide Repeats in Fragile X Mental Retardation-1 Gene

A Robust Polymerase Chain Reaction-based Assay for Quantifying Cytosine-guanine-guanine Trinucleotide Repeats in Fragile X Mental Retardation-1 Gene

Published on: September 16, 2019

Related Experiment Videos

Last Updated: May 1, 2026

Chromatin Interaction Analysis with Paired-End Tag Sequencing ChIA-PET for Mapping Chromatin Interactions and Understanding Transcription Regulation

Chromatin Interaction Analysis with Paired-End Tag Sequencing ChIA-PET for Mapping Chromatin Interactions and Understanding Transcription Regulation

Published on: April 30, 2012

Identifying Mutations by High Resolution Melting in a TILLING Population of Rice

Identifying Mutations by High Resolution Melting in a TILLING Population of Rice

Published on: September 2, 2019

A Robust Polymerase Chain Reaction-based Assay for Quantifying Cytosine-guanine-guanine Trinucleotide Repeats in Fragile X Mental Retardation-1 Gene

A Robust Polymerase Chain Reaction-based Assay for Quantifying Cytosine-guanine-guanine Trinucleotide Repeats in Fragile X Mental Retardation-1 Gene

Published on: September 16, 2019

Area of Science:

Botany
Molecular Biology
Pharmacognosy

Background:

The "Chirayat" complex includes various Swertia species and non-Swertia species used as substitutes or adulterants for Swertia chirayita.
Accurate identification of these species is crucial for quality control and therapeutic efficacy.

Purpose of the Study:

To characterize 12 taxa within the "Chirayat" complex using Inter-Simple Sequence Repeat (ISSR) markers.
To evaluate the efficiency of ISSR markers for the molecular authentication of medicinal plants and commercial crude drug samples.

Main Methods:

DNA was extracted from 12 different taxa within the "Chirayat" complex.
Inter-Simple Sequence Repeat (ISSR) markers were employed for molecular profiling.
Preliminary screening identified 16 informative primers, with a focus on those with 5' end anchors for polymorphic profiles.
Cluster analysis was performed based on ISSR marker data to assess genetic relationships.

Main Results:

All 16 ISSR primers generated distinct and reproducible DNA amplification profiles.
A total of 332 polymorphic bands were scored, indicating high genetic variability.
Primer 885 exhibited the highest resolving power for distinguishing taxa.
Cluster analysis revealed two main clusters within the Swertia species, with Swertia lurida showing the closest genetic similarity to Swertia chirata.

Conclusions:

ISSR markers are highly efficient and reliable for the molecular authentication of individual taxa within the "Chirayat" complex.
This method can be effectively used to authenticate commercial crude drug samples, ensuring quality and preventing adulteration.
ISSR profiling provides a robust tool for pharmacognostic studies of medicinal plants.