Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

DNA Isolation

DNA Isolation

DNA isolation protocols can be fast and straightforward or complex and time-consuming depending on the type and quality of DNA required for further processing. For example, plasmid DNA extraction is a bit more complicated than genomic DNA extraction because of the need for an appropriate lysis method to separate plasmid DNA from gDNA during isolation. However, for specific applications, such as long-range DNA sequencing that require a good yield of high- quality DNA samples, we need to follow...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Understanding barriers to treatment-seeking in Mseleni joint disease: A multistaged study in rural KwaZulu-Natal, South Africa.

African journal of primary health care & family medicine·2026

Same author

Combinatorial drug repurposing of Valproic acid and Zebularine regulates Krüppel-like factor 4 and β-catenin expression in colon cancer cells.

PloS one·2026

Same author

Evidence for an ethics-practice gap in digital anatomy education: Provenance and consent disclosure in English-language YouTube videos.

Annals of anatomy = Anatomischer Anzeiger : official organ of the Anatomische Gesellschaft·2026

Same author

Further considerations on the international transfer of donated bodies: The environmental impact.

Anatomical sciences education·2026

Same author

Forensic taphonomy and stable isotopes: δ<sup>13</sup>C and δ<sup>15</sup>N enrichment patterns in human skin and muscle show promise for future forensic applications.

International journal of legal medicine·2026

Same author

A generalizable cross-continent prediction of esophageal squamous cell carcinoma using the oral microbiome.

Communications medicine·2026

Search research articles

Related Experiment Video

Updated: Jun 24, 2026

Optimized Bone Sampling Protocols for the Retrieval of Ancient DNA from Archaeological Remains

Optimized Bone Sampling Protocols for the Retrieval of Ancient DNA from Archaeological Remains

Published on: November 30, 2021

Brief communication: Minimally invasive bone sampling method for DNA analysis.

Victoria E Gibbon¹, Clem B Penny, Goran Strkalj

¹Department of Internal Medicine, University of the Witwatersrand, Johannesburg, Parktown, Johannesburg 2193, South Africa. gibbonv@gmail.com

American Journal of Physical Anthropology

|April 8, 2009

Summary

This summary is machine-generated.

A new minimally invasive bone sampling technique allows DNA analysis without damaging crucial skeletal landmarks. This method ensures sample integrity and is safe for rare human and animal specimens.

More Related Videos

Absorbent Microbiopsy Sampling and RNA Extraction for Minimally Invasive, Simultaneous Blood and Skin Analysis

Absorbent Microbiopsy Sampling and RNA Extraction for Minimally Invasive, Simultaneous Blood and Skin Analysis

Published on: February 21, 2019

Methods to Enable Spatial Transcriptomics of Bone Tissues

Methods to Enable Spatial Transcriptomics of Bone Tissues

Published on: May 3, 2024

Related Experiment Videos

Last Updated: Jun 24, 2026

Optimized Bone Sampling Protocols for the Retrieval of Ancient DNA from Archaeological Remains

Optimized Bone Sampling Protocols for the Retrieval of Ancient DNA from Archaeological Remains

Published on: November 30, 2021

Absorbent Microbiopsy Sampling and RNA Extraction for Minimally Invasive, Simultaneous Blood and Skin Analysis

Absorbent Microbiopsy Sampling and RNA Extraction for Minimally Invasive, Simultaneous Blood and Skin Analysis

Published on: February 21, 2019

Methods to Enable Spatial Transcriptomics of Bone Tissues

Methods to Enable Spatial Transcriptomics of Bone Tissues

Published on: May 3, 2024

Area of Science:

Paleoproteomics
Forensic Anthropology
Molecular Biology

Background:

Traditional bone sampling for DNA analysis is destructive.
Curators are hesitant to permit invasive testing on skeletal collections.
Need for non-destructive methods for ancient DNA extraction.

Purpose of the Study:

Introduce a novel, minimally invasive bone sampling method for DNA analysis.
Assess the impact of the sampling method on sample integrity.
Evaluate the applicability of the method to diverse skeletal materials.

Main Methods:

Utilized a conventional hand drill for bone sample extraction.
Targeted the intercondylar fossa of the femur to avoid anthropometric landmarks.
Monitored drill temperature to ensure molecular integrity.

Main Results:

The novel method leaves only a small surface hole, preserving landmarks.
Minimal temperature increase during drilling does not compromise DNA.
The technique is easily replicable and suitable for various skeletal remains.

Conclusions:

This minimally invasive technique facilitates DNA analysis from skeletal collections.
It minimizes risk to valuable and rare specimens.
Enables wider application of molecular analyses in anthropology and paleontology.