Related Experiment Video
Updated: Jun 24, 2026
14:48
Proteomic Profile of EPS-Urine through FASP Digestion and Data-Independent Analysis
Published on: May 8, 2021
Should urine pH be adjusted prior to gel-based proteome analysis?
Visith Thongboonkerd1, Siriwan Mungdee, Wararat Chiangjong
1Medical Proteomics Unit, Office for Research and Development, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand. thongboonkerd@dr.com
Journal of Proteome Research
|April 9, 2009
Summary
Urine pH variation does not significantly impact urinary proteome profiles. Adjustment of urine pH is unnecessary before gel-based proteome analysis, simplifying biomarker discovery workflows.
Area of Science:
- Clinical Chemistry
- Proteomics
- Biomarker Discovery
Background:
- Urine is a key clinical sample for biomarker discovery.
- Human urine pH varies widely (4.5-8.0).
- The impact of urine pH on proteome analysis was previously unclear.
Purpose of the Study:
- To systematically analyze the effect of urine pH on urinary proteome profiles.
- To determine if urine pH adjustment is necessary before proteome analysis.
Main Methods:
- Collected urine samples from healthy individuals.
- Isolated urinary proteins using ethanol precipitation.
- Analyzed proteome profiles using 2-DE and SYPRO Ruby staining.
- Adjusted pooled urine pH to assess its effect on proteome profiles.
Main Results:
- No significant correlation found between urine pH differences and proteome spot matching in individual samples.
- No significant differences in detected spots or %match across various pH levels in pooled samples.
- Statistical analysis (ANOVA, Tukey's test) confirmed no pH-dependent proteome variations.
Conclusions:
- Urine pH does not significantly affect urinary proteome profiles.
- No adjustment of urine pH is required before gel-based proteome analysis.
- This finding simplifies sample preparation for urinary biomarker discovery.
