Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Microbial Biosensors01:17

Microbial Biosensors

Microbial biosensors are analytical devices that utilize living microbes to detect specific substances through measurable signals. These devices consist of two main components: biosensing organisms and signal-transducing elements. Biosensing organisms, such as Escherichia coli or Saccharomyces cerevisiae, are typically housed in multiwell plates connected to transducers, enabling rapid, real-time detection of target analytes.Signal Generation MechanismWhen a target analyte—such as...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Scaling Nanophotonic Interferometric Biosensors toward Parallel Detection of Multiple Biomarkers.

ACS sensors·2026
Same author

Multi-omic profiling reveals pericyte and smooth muscle cell contributions to CADASIL pathology in cell-specific Notch3 mutant mice.

Cell reports·2026
Same author

Interrelationships Among Health-Related Social Needs: Strong Clustering Patterns Identified Through Comprehensive Screening.

Journal of general internal medicine·2026
Same author

Dementia Care Research and Psychosocial Factors.

Alzheimer's & dementia : the journal of the Alzheimer's Association·2025
Same author

Epitope mapping of SARS-CoV-2 Spike protein using naturally-acquired immune responses to develop monoclonal antibodies.

Scientific reports·2025
Same author

Identification and ultrasensitive quantification of H. pylori infections on gastric and stool human samples with a photonic label-free nanobiosensor.

Biosensors & bioelectronics·2025
Same journal

The ACS at 150: The History of Analytical Chemistry Publications and a Century of Progress.

Analytical chemistry·2026
Same journal

Machine Learning-Enabled Image Analysis of Complex Chemical Mixtures: Synthetic Urine Droplets as a Test System.

Analytical chemistry·2026
Same journal

H<sub>2</sub>O<sub>2</sub>/Viscosity Tandem-Locked Fluorescent Probes Based on an In Situ Fluorophore Synthesis Strategy for Colitis Imaging and Diagnosis.

Analytical chemistry·2026
Same journal

TopoStitcher: A Geometric-Topological Structure-Guided Stitching Framework for Single-Molecule Localization Microscopy.

Analytical chemistry·2026
Same journal

Noninvasive SERS Immunosensing of Tyrosinase for Melanoma Monitoring via Microneedle Sampling Integrated with Satellite-Structured Bifunctional Nanozymes.

Analytical chemistry·2026
Same journal

Label-Free Electrochemical CRISPR Platform Gated by Allosteric Transcription Factors for Ultrasensitive Small-Molecule Detection.

Analytical chemistry·2026
See all related articles

Related Experiment Video

Updated: Jun 24, 2026

DNAzyme 10-23 - Based Nanomachines for Nucleic Acid Recognition
07:16

DNAzyme 10-23 - Based Nanomachines for Nucleic Acid Recognition

Published on: February 9, 2024

Locked nucleic acid based beacons for surface interaction studies and biosensor development.

Karen Martinez1, M-Carmen Estevez, Yanrong Wu

  • 1Center for Research at the Bio/Nano Interface, Department of Chemistry, University of Florida, Gainesville, Florida 32611, USA.

Analytical Chemistry
|April 9, 2009
PubMed
Summary
This summary is machine-generated.

Researchers developed a new locked nucleic acid base (LNA) molecular beacon (MB) for enhanced DNA detection on surfaces. This LNA molecular beacon (LMB) shows improved stability and a 25-fold signal enhancement compared to regular MBs.

More Related Videos

Analyzing Dynamic Protein Complexes Assembled On and Released From Biolayer Interferometry Biosensor Using Mass Spectrometry and Electron Microscopy
09:30

Analyzing Dynamic Protein Complexes Assembled On and Released From Biolayer Interferometry Biosensor Using Mass Spectrometry and Electron Microscopy

Published on: August 6, 2018

Exploring Biomolecular Interaction Between the Molecular Chaperone Hsp90 and Its Client Protein Kinase Cdc37 using Field-Effect Biosensing Technology
09:39

Exploring Biomolecular Interaction Between the Molecular Chaperone Hsp90 and Its Client Protein Kinase Cdc37 using Field-Effect Biosensing Technology

Published on: March 31, 2022

Related Experiment Videos

Last Updated: Jun 24, 2026

DNAzyme 10-23 - Based Nanomachines for Nucleic Acid Recognition
07:16

DNAzyme 10-23 - Based Nanomachines for Nucleic Acid Recognition

Published on: February 9, 2024

Analyzing Dynamic Protein Complexes Assembled On and Released From Biolayer Interferometry Biosensor Using Mass Spectrometry and Electron Microscopy
09:30

Analyzing Dynamic Protein Complexes Assembled On and Released From Biolayer Interferometry Biosensor Using Mass Spectrometry and Electron Microscopy

Published on: August 6, 2018

Exploring Biomolecular Interaction Between the Molecular Chaperone Hsp90 and Its Client Protein Kinase Cdc37 using Field-Effect Biosensing Technology
09:39

Exploring Biomolecular Interaction Between the Molecular Chaperone Hsp90 and Its Client Protein Kinase Cdc37 using Field-Effect Biosensing Technology

Published on: March 31, 2022

Area of Science:

  • Biotechnology
  • Molecular Biology
  • Biosensor Technology

Background:

  • Molecular beacons (MBs) are used for nucleic acid detection but suffer from poor stability and low signal enhancement when immobilized on solid surfaces.
  • The hairpin structure of conventional MBs limits their effectiveness in surface-based biosensor applications.

Purpose of the Study:

  • To design and evaluate a novel molecular beacon incorporating locked nucleic acid bases (LNAs) for improved surface immobilization and DNA detection.
  • To compare the performance of the LNA molecular beacon (LMB) against regular molecular beacons (RMBs) in terms of stability, sensitivity, and robustness.

Main Methods:

  • Designed and synthesized LNA-modified molecular beacons (LMBs).
  • Immobilized LMBs and RMBs onto solid surfaces for DNA detection.
  • Evaluated and compared selectivity, sensitivity, thermal stability, hybridization kinetics, and robustness of LMBs and RMBs.

Main Results:

  • LMBs demonstrated significantly enhanced stability and robustness upon surface immobilization compared to RMBs.
  • A 25-fold signal enhancement was achieved with LMBs, reaching detection limits in the low nanomolar range.
  • LMB-based biosensors exhibited superior reproducibility and selectivity over RMB-based systems.

Conclusions:

  • Locked nucleic acid bases (LNAs) improve the stability and performance of molecular beacons for surface immobilization.
  • LNA molecular beacons (LMBs) offer a promising alternative to conventional DNA probes for DNA microarrays and biosensors.
  • The developed LMBs provide a robust and sensitive platform for real-time nucleic acid detection.