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Related Concept Videos

Double Resonance Techniques: Overview01:12

Double Resonance Techniques: Overview

Double resonance techniques in Nuclear Magnetic Resonance (NMR) spectroscopy involve the simultaneous application of two different frequencies or radiofrequency pulses to manipulate and observe two distinct nuclear spins. One important application of double resonance is spin decoupling, which selectively suppresses coupling with one type of nucleus while observing the NMR signal from another nucleus, simplifying the spectrum and enhancing resolution.
Spin decoupling is usually achieved by...
¹H NMR: Interpreting Distorted and Overlapping Signals01:02

¹H NMR: Interpreting Distorted and Overlapping Signals

Spin systems where the difference in chemical shifts of the coupled nuclei is greater than ten times J are called first-order spin systems. These nuclei are weakly coupled, and their chemical shifts and coupling constant can generally be estimated from the well-separated signals in the spectrum.
As Δν decreases and the signals move closer, the doublets appear increasingly distorted. The intensities of the inner lines increase at the cost of those of the outer lines as the signals are slanted or...
NMR Spectroscopy: Spin–Spin Coupling01:08

NMR Spectroscopy: Spin–Spin Coupling

The spin state of an NMR-active nucleus can have a slight effect on its immediate electronic environment. This effect propagates through the intervening bonds and affects the electronic environments of NMR-active nuclei up to three bonds away; occasionally, even farther. This phenomenon is called spin–spin coupling or J-coupling. Coupling interactions are mutual and result in small changes in the absorption frequencies of both nuclei involved. While nuclei of the same element are involved in...
2D NMR: Overview of Homonuclear Correlation Techniques01:16

2D NMR: Overview of Homonuclear Correlation Techniques

Homonuclear correlation spectroscopy (COSY) is a powerful technique used in Nuclear Magnetic Resonance (NMR) spectroscopy to study the correlations between nuclei of the same type within a molecule. It provides information about scalar couplings between adjacent nuclei, which helps determine connectivity and structural information. There are several COSY variants, each with its unique strengths and experimental parameters.
COSY90 is the standard two-dimensional (2D) COSY experiment that...
2D NMR: Overview of Heteronuclear Correlation Techniques01:18

2D NMR: Overview of Heteronuclear Correlation Techniques

Heteronuclear correlation spectroscopy is an analytical technique that investigates the coupling between different types of nuclei, often a proton and an X-nucleus, such as carbon-13 or nitrogen-15. This method is commonly used in nuclear magnetic resonance (NMR) spectroscopy to gain insights into complex chemical compounds' structural and compositional aspects. A typical heteronuclear correlation spectrum displays X-nucleus chemical shifts on one axis and a proton spectrum on the other axis.
NMR Spectrometers: Resolution and Error Correction01:14

NMR Spectrometers: Resolution and Error Correction

When magnetic nuclei in a sample achieve resonance and undergo relaxation, the signal detected in NMR is an approximately exponential free induction decay. Fourier transform of an exponential decay yields a Lorentzian peak in the frequency domain. Lorentzian peaks in an NMR spectrum are defined by their amplitude, full width at half maximum, and position, where the peak width is governed by the spin-spin relaxation time alone. In real experiments, however, the applied magnetic field is rendered...

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Atomic Scale Structural Studies of Macromolecular Assemblies by Solid-state Nuclear Magnetic Resonance Spectroscopy
14:55

Atomic Scale Structural Studies of Macromolecular Assemblies by Solid-state Nuclear Magnetic Resonance Spectroscopy

Published on: September 17, 2017

Sensitivity enhancement in static solid-state NMR experiments via single- and multiple-quantum dipolar coherences.

T Gopinath1, Gianluigi Veglia

  • 1Department of Chemistry, University of Minnesota, Minneapolis, Minnesota 55455, USA.

Journal of the American Chemical Society
|April 9, 2009
PubMed
Summary

A novel method boosts static solid-state NMR sensitivity by up to 40% using combined quantum coherences. This technique enhances signal-to-noise ratio for crucial applications in liquid crystals and membrane proteins.

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Atomic Scale Structural Studies of Macromolecular Assemblies by Solid-state Nuclear Magnetic Resonance Spectroscopy
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NMR 15N Relaxation Experiments for the Investigation of Picosecond to Nanoseconds Structural Dynamics of Proteins

Published on: November 1, 2024

Area of Science:

  • Solid-state Nuclear Magnetic Resonance (NMR) spectroscopy.
  • Biophysical chemistry and structural biology.

Background:

  • Static solid-state NMR experiments often face limitations in sensitivity, hindering detailed structural analysis.
  • Existing sensitivity enhancement schemes are primarily developed for solution-state NMR and are not directly applicable to solid-state systems.

Purpose of the Study:

  • To develop a new method for significantly enhancing sensitivity in static solid-state NMR experiments.
  • To improve the signal-to-noise ratio (SNR) for more robust structural determination of challenging samples.

Main Methods:

  • A novel approach combining single- and multiple-quantum dipolar coherences was implemented.
  • The method was demonstrated using the polarization inversion spin exchange at magic angle (PISEMA) experiment.
  • The technique's generalizability to other separated local field experiments was explored.

Main Results:

  • Achieved a sensitivity gain of up to 40% in static solid-state NMR experiments.
  • Demonstrated a distinct mechanism for sensitivity enhancement compared to solution NMR methods.
  • Validated the applicability of the method to PISEMA and its potential for broader use.

Conclusions:

  • The developed method offers a substantial improvement in SNR for static solid-state NMR.
  • This technique is distinct from solution NMR approaches and tailored for solid-state systems.
  • The method holds significant promise for advancing the study of aligned liquid crystals and membrane proteins in lipid membranes.