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[Lethal effect of cytotoxin from Agkitrodon acutus venom on gastric cancer cells and their ultrastructure changes]
1Department of Gastrointestinal Surgery, Affiliated Hospital of Guangdong Medical College, Zhanjiang 524000, China.
Aim:
To purify cytotoxin from Agkitrodon acutus venom, and study the lethal effect of cytotoxin on gastric cancer cells and their ultrastructure changes.
Methods:
The cytotoxin was isolated and purified with successive chromatography on DEAE-Sephadex A-50 and TSKgel Pheny columns from Agkitrodon acutus venom. The feasibility of BGC-823 cells assessed using the MTT assay. The ultrastructure of BGC-823 cells treated with the cytotoxin was observed with the scanning and transmission electron microscope.
Results:
The purified cytotoxin H1 killed the gastric cancer cells. The cancer cell membrane and nuclear envelope were broken, mitochondria were swollen and intracellular substances were leaked out.
Conclusion:
The cytotoxin from Agkitrodon acutus venom can kill the gastric cancer cells effectively.
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