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Related Concept Videos

Production of Organic Acids01:25

Production of Organic Acids

Lactic acid, an important organic acid extensively applied in food, pharmaceutical, and biodegradable polymer industries, is primarily produced via microbial fermentation. This method is favored over chemical synthesis due to its environmental sustainability and capacity for enantiomerically pure product formation. Among various microbial processes, the fermentation of starch-based substrates stands out due to the abundance and renewability of raw materials like corn and potatoes.Hydrolysis of...
Microbial Fermentation01:23

Microbial Fermentation

Fermentation is a crucial anaerobic metabolic process that enables microbes to derive energy from sugar without relying on oxygen or an electron transport chain. This process is fundamental to various biological and industrial applications and is classified based on the metabolic products generated.Role of Pyruvate in FermentationPyruvate and its derivatives serve as key electron acceptors in fermentative pathways. The oxidation of NADH to regenerate NAD+ is essential for the continuation of...
Microbes in the Production of Fermented Foods01:27

Microbes in the Production of Fermented Foods

Lactic acid bacteria (LAB) and molds are instrumental in fermenting plant-based foods to enhance preservation and ensure year-round availability. These microbial processes convert plant carbohydrates into organic acids and other metabolites that inhibit spoilage organisms and contribute to the sensory qualities of the final product.In sauerkraut production, cabbage goes through a microbial succession that starts with cocci such as Leuconostoc mesenteroides. These microbes begin fermentation by...
Microbial Corrosion01:24

Microbial Corrosion

Microbiologically Influenced Corrosion (MIC) is a significant form of material degradation caused by the metabolic activities of microorganisms. This phenomenon poses substantial challenges across various industries, including oil and gas, maritime, and water treatment sectors.MIC occurs when microorganisms, such as bacteria, archaea, and fungi, colonize metal surfaces, forming biofilms that alter the local electrochemical environment. These biofilms can lead to the production of corrosive...
Loss of Carboxy Group as CO2: Decarboxylation of Malonic Acid Derivatives01:35

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Just like β-keto acids—which upon thermal decarboxylation form ketones—β-dicarboxylic acids undergo decarboxylation to generate monocarboxylic acids with the liberation of carbon dioxide.
Polyprotic Acids03:38

Polyprotic Acids

Acids are classified by the number of protons per molecule that they can give up in a reaction. Acids such as HCl, HNO3, and HCN that contain one ionizable hydrogen atom in each molecule are called monoprotic acids. Their reactions with water are:

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Related Experiment Video

Updated: Jun 24, 2026

A Rapid and Specific Microplate Assay for the Determination of Intra- and Extracellular Ascorbate in Cultured Cells
11:56

A Rapid and Specific Microplate Assay for the Determination of Intra- and Extracellular Ascorbate in Cultured Cells

Published on: April 11, 2014

Microbial conversion of L-ascorbic acid to L-erythroascorbic acid.

Daiki Kyotani1, Keiko Hasegawa, Hideo Ohishi

  • 1Department of Applied Biological Science, Tokyo University of Agriculture and Technology, 3-5-8 Saiwaicho, Fuchu, Tokyo 183-8509, Japan.

Bioscience, Biotechnology, and Biochemistry
|April 9, 2009
PubMed
Summary
This summary is machine-generated.

A Penicillium sp. strain efficiently converts L-ascorbic acid into L-erythroascorbic acid, a valuable five-carbon analog. This bioconversion process, utilizing growing cultures or washed mycelia, offers a novel method for producing this important compound.

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Published on: December 12, 2013

Area of Science:

  • Biotechnology
  • Microbiology
  • Organic Chemistry

Background:

  • L-ascorbic acid (Vitamin C) is a vital nutrient with various industrial applications.
  • Exploring microbial bioconversion pathways can lead to novel synthesis methods for valuable compounds.
  • L-erythroascorbic acid is a five-carbon analog of L-ascorbic acid with potential applications.

Purpose of the Study:

  • To investigate the microbial conversion of L-ascorbic acid to its five-carbon analog.
  • To identify the resulting product using spectroscopic analysis.
  • To describe the bioconversion and purification processes.

Main Methods:

  • Utilizing a specific strain of Penicillium sp. for bioconversion.
  • Employing growing cultures and washed mycelia as biocatalysts.
  • Spectroscopic analysis for product identification and yield determination.

Main Results:

  • Penicillium sp. effectively converted L-ascorbic acid to L-erythroascorbic acid.
  • The yield of L-erythroascorbic acid was approximately 20-30% (mol/mol).
  • Spectroscopic analysis confirmed the identity of the product as L-erythroascorbic acid.

Conclusions:

  • Microbial bioconversion using Penicillium sp. is a viable method for producing L-erythroascorbic acid.
  • The study details the bioconversion and purification processes for practical application.
  • This research contributes to the development of sustainable synthesis routes for vitamin C analogs.