Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Induced Pluripotent Stem Cells

Induced Pluripotent Stem Cells

Stem cells are undifferentiated cells that divide and produce different cell types. Ordinarily, cells that have differentiated into a specific cell type are terminally differentiated; however, scientists have found a way to reprogram these mature cells so that they dedifferentiate and return to an unspecialized, proliferative state. These cells are pluripotent like embryonic stem cells—able to produce all cell types—and are called induced pluripotent stem cells (iPSCs).
Somatic cells are...

Induced Pluripotent Stem Cells

Induced Pluripotent Stem Cells

Stem cells are undifferentiated cells that divide and produce different types of cells. Ordinarily, cells that have differentiated into a specific cell type are post-mitotic—that is, they no longer divide. However, scientists have found a way to reprogram these mature cells so that they “de-differentiate” and return to an unspecialized, proliferative state. These cells are also pluripotent like embryonic stem cells—able to produce all cell types—and are therefore called induced pluripotent stem...

Induced Pluripotent Stem Cells

Induced Pluripotent Stem Cells

Stem cells are undifferentiated cells that divide and produce different types of cells. Ordinarily, cells that have differentiated into a specific cell type are post-mitotic—that is, they no longer divide. However, scientists have found a way to reprogram these mature cells so that they “de-differentiate” and return to an unspecialized, proliferative state. These cells are also pluripotent like embryonic stem cells—able to produce all cell types—and are therefore called induced pluripotent stem...

Stem Cell Culture

Stem Cell Culture

Stem cell research aims to find ways to use stem cells to regenerate and repair cellular damage. Over time, most adult cells undergo the wear and tear of aging and lose their ability to divide and repair themselves. Stem cells do not display a particular morphology or function. Adult stem cells, which exist as a small subset of cells in most tissues, keep dividing and can differentiate into a number of specialized cells generally formed by that tissue. These cells enable the body to renew and...

iPS Cell Differentiation

iPS Cell Differentiation

The ability of induced pluripotent stem cells or iPSCs to differentiate into most body cell types has stimulated repair and regenerative medicine research over the past few decades. iPSC-derived blood cells, hepatocytes, beta islet cells, cardiomyocytes, neurons, and other cell types can repair injuries or regenerate damaged tissue in diseases such as diabetes and neurodegenerative disorders.

EPS and iPS Cells in Disease Research

EPS and iPS Cells in Disease Research

Embryonic and induced pluripotent stem cells are excellent models for disease research because of their ability to self-renew and differentiate into most cell types. Somatic cells from a patient are isolated and reprogrammed into induced pluripotent stem cells or iPSCs. These iPSCs are later differentiated into the desired cell type, which mirrors the diseased cell of the patient. In this way, disease models have been created for investigating diseases such as Down syndrome, type I diabetes,...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Standard-of-care ciltacabtagene autoleucel in earlier versus later lines of therapy for relapsed or refractory multiple myeloma: a nationwide registry analysis.

Journal of hematology & oncology·2026

Same author

Human CART22.19 therapy in refractory pediatric B-ALL: insights from a named-patient cohort.

Journal for immunotherapy of cancer·2026

Same author

Characterizing the impact of MLL fusion variants and fusion partners on leukemia plasticity using a human CRISPR-engineered MLL-rearranged leukemia model.

Neoplasia (New York, N.Y.)·2026

Same author

Anti CD19 targeting CAR T cell therapy in ANCA-associated vasculitis.

Rheumatology (Oxford, England)·2026

Same author

Predictive capacity of peritransplant measurable residual disease thresholds in NPM1-mutant acute myeloid leukemia.

Blood advances·2026

Same author

Real world outcomes of intravitreal and systemic therapy in primary and secondary vitreoretinal lymphoma.

Scientific reports·2026

Search research articles

Related Experiment Video

Updated: Jun 24, 2026

Scalable 96-well Plate Based iPSC Culture and Production Using a Robotic Liquid Handling System

Scalable 96-well Plate Based iPSC Culture and Production Using a Robotic Liquid Handling System

Published on: May 14, 2015

Patient-specific pluripotent stem cells: promises and challenges.

Rouven Müller¹, Claudia Lengerke

¹Laboratory for Developmental Biology, Department of Hematology and Oncology, University of Tübingen Medical Center II, Tübingen, Germany.

Nature Reviews. Endocrinology

|April 9, 2009

Summary

Generating pluripotent stem cells from adult somatic cells offers a promising alternative to embryonic stem cells for regenerative medicine. This approach bypasses ethical concerns and provides patient-specific cells, advancing cell-based therapies.

More Related Videos

Reprogramming Induced Pluripotent Stem Cell Lines from Frozen Buffy Coat Samples

Reprogramming Induced Pluripotent Stem Cell Lines from Frozen Buffy Coat Samples

Published on: April 10, 2026

Related Experiment Videos

Last Updated: Jun 24, 2026

Scalable 96-well Plate Based iPSC Culture and Production Using a Robotic Liquid Handling System

Scalable 96-well Plate Based iPSC Culture and Production Using a Robotic Liquid Handling System

Published on: May 14, 2015

Reprogramming Induced Pluripotent Stem Cell Lines from Frozen Buffy Coat Samples

Reprogramming Induced Pluripotent Stem Cell Lines from Frozen Buffy Coat Samples

Published on: April 10, 2026

Area of Science:

Regenerative Medicine
Stem Cell Biology
Immunology

Background:

Tissue transplantation is vital for treating degenerative and malignant disorders but faces challenges with donor compatibility and tissue availability.
Human embryonic stem cells (hESCs) offered potential for universal cell-based treatments, but their clinical application is limited by technical and ethical issues.
Patient-specific hESCs derived via somatic-cell nuclear transfer or parthenogenesis can avoid immunosuppression but still involve ethical considerations.

Purpose of the Study:

To critically analyze current methods for generating pluripotent stem cells.
To discuss the clinical prospects of pluripotent stem cells derived from reprogrammed adult somatic cells.
To explore alternatives to embryonic stem cells for cell-based therapies.

Main Methods:

Review of existing literature on pluripotent stem cell generation techniques.
Analysis of reprogramming methods for adult somatic cells into induced pluripotent stem cells (iPSCs).
Evaluation of ethical considerations and technical hurdles associated with different stem cell sources.

Main Results:

Reprogramming adult somatic cells provides a viable method for generating patient-specific pluripotent stem cell lines.
This approach circumvents the ethical concerns and technical limitations associated with embryonic stem cells.
Induced pluripotent stem cells can be derived without progression through the blastocyst stage.

Conclusions:

Reprogrammed adult somatic cells represent a significant advancement in stem cell therapy, offering a safer and more accessible alternative.
Patient-specific pluripotent stem cells hold great promise for personalized regenerative medicine, potentially eliminating the need for immunosuppression.
Further research and clinical translation are needed to fully realize the therapeutic potential of these stem cell technologies.