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Updated: Jun 24, 2026

Flow Cytometric Analysis of Biomarkers for Detecting Human Sperm Functional Defects
Published on: April 21, 2022
The sperm chromatin structure assay (SCSA) as prognostic factor in IVF/ICSI program
Piotr Miciński1, Krzysztof Pawlicki, Ewa Wielgus
1Novomedica, ul. Bończyka 34, 41-400 Mysłowice, Poland. micinski@ka.onet.pl
Abstract:
In this study 60 couples undergoing intracytoplasmic sperm injection (ICSI) procedures were enrolled. All men were classified into two groups regarding to the DNA fractionation index (DFI) threshold value: group I < 15% and group II =or> 15%. In group I, median DFI was 4%, normal pre-implantation embryo development was observed and eleven pregnancies were achieved. In group II, median DFI was 23% and normal pre-implantation embryo development was also observed, but only two pregnancies were achieved. Our results suggest that the patients included in the assisted reproductive techniques (ART) should be diagnosed with the SCSA test and the DFI may be related to the outcome of fertilization process as well as to the number of transferred embryos and pregnancy.
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