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Grape and wine proteins: their fractionation by hydrophobic interaction chromatography and identification by

Matteo Marangon1, Steven C Van Sluyter, Paul A Haynes

  • 1Dipartimento di Biotecnologie agrarie and Centro Interdipartimentale per la Ricerca in Viticoltura ed Enologia, Università di Padova, via dell'Università 16, I-35020 Legnaro, PD, Italy.

Journal of Agricultural and Food Chemistry
|April 10, 2009
PubMed

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The stability and compatibility of column material with samples are crucial for efficient purification in chromatographic techniques. Various operating parameters such as pH, temperature, or solvent affect the packing of the column material, thereby determining the purification efficiency. The choice of column material also plays an essential role in deciding the operating parameters and can be modified based on the proteins that need to be purified.
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