Related Experiment Video
Updated: Jun 24, 2026
08:26
Refinement of OnePot PURE and Crude Ribosome Production for Reproducible Cell-free Protein Synthesis
Published on: August 22, 2025
Design and synthesis of substrates for model ribosomal reactions
Stanislav G Bayryamov1, Nikolay G Vassilev, Miroslav A Rangelov
1Laboratory of BioCatalysis, Institute of Organic Chemistry, Bulgarian Academy of Sciences, Sofia 1113, Bulgaria. stanly_chem2002@yahoo.com
Protein and Peptide Letters
|April 10, 2009
Abstract:
2'/3'-O-[Bz(NO(2))-Orn(Boc)]-5'-O-Piv-Ado (1) and its deoxy analog: 3'-O-[Bz(NO(2))-Orn(Boc)]-5'-O-Piv-2'-dAdo (2) were designed and synthesized as substrates for the model ribosome reaction we used to demonstrate the crucial role of A76 2'-OH of peptidyl-tRNA in the rate acceleration of peptide bond formation during protein biosynthesis.
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