Optical biopsy using spectral camera in BCC and oral leukoplakia

Area of Science:

• Oncology
• Dermatology
• Medical Diagnostics
• Biophotonics

Background:

• Basal cell carcinoma (BCC) and oral leukoplakia are common skin neoplasms.
• Early diagnosis of BCC and oral leukoplakia significantly improves treatment outcomes.
• Protoporphyrin IX (PPIX) is a photosensitizer relevant to tumor diagnostics.

Purpose of the Study:

• To evaluate the efficacy of fluorescence spectroscopy in diagnosing BCC and oral leukoplakia.
• To investigate the diagnostic potential of protoporphyrin IX (PPIX) fluorescence at 630 nm.
• To compare spectral camera findings with the Xillix Onco Life system.

Main Methods:

• Utilized a spectral camera with filters (570-650 nm) to measure fluorescence spectra.
• Administered delta-aminolevulinic acid topically 8 hours prior to examination.
• Compared spectral data from neoplastic tissues with healthy contralateral tissues.
• Correlated spectral camera findings with Xillix Onco Life numerical color value (NCV).

Main Results:

• A significant fluorescence emission peak at 630 nm was observed in BCC and oral leukoplakia tissues.
• Healthy tissues showed no distinct 630 nm peak, with decreasing fluorescence intensity from 570 nm.
• The intensity of 630 nm fluorescence strongly correlated with Xillix Onco Life NCV.
• The spectral camera successfully differentiated neoplastic from healthy tissues.

Conclusions:

• Fluorescence spectroscopy, particularly detecting the 630 nm PPIX peak, is a promising objective method for early diagnosis of BCC and oral leukoplakia.
• This technique offers a non-invasive approach to identify cancerous and precancerous skin lesions.
• The findings support the clinical utility of fluorescence spectroscopy in dermatological oncology.