Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Classical and alternative complement pathway activation by pneumococci.

C G Stephens, R C Williams, W P Reed

    Infection and Immunity
    |August 1, 1977
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Studies of antibody to herpes simplex virus Fc gamma-binding protein gE in patients with rheumatoid arthritis, juvenile rheumatoid arthritis and normal controls.

    Scandinavian journal of immunology·1992
    Same author

    Radiographic evaluation of crestal bone levels adjacent to nonsubmerged titanium implants.

    Clinical oral implants research·1992
    Same author

    Serologic cross-reactivities poorly reflect allelic relationships in the HLA-B12 and HLA-B21 groups. Dominant epitopes of the alpha 2 helix.

    Journal of immunology (Baltimore, Md. : 1950)·1992
    Same author

    Human anti-F(ab')2 antibodies show preferential reactivity for F(ab')2 molecules bearing lambda light chains.

    Clinical immunology and immunopathology·1992
    Same author

    Differential mapping of Fc gamma-binding and monoclonal antibody-reactive epitopes on gE, the Fc gamma-binding glycoprotein of herpes simplex virus type 1.

    Journal of immunology (Baltimore, Md. : 1950)·1992
    Same author

    Heteroclitic polyclonal and monoclonal anti-Gm(a) and anti-Gm(g) human rheumatoid factors react with epitopes induced in Gm(a-), Gm(g-) IgG by interaction with antigen or by nonspecific aggregation. A possible mechanism for the in vivo generation of rheumatoid factors.

    Journal of immunology (Baltimore, Md. : 1950)·1992
    Same journal

    The cholesterol-dependent cytolysin promotes <i>Streptococcus</i> systemic spread and induces arachidonic acid accumulation-mediated lethality in a murine intraperitoneal infection model.

    Infection and immunity·2026
    Same journal

    Phenotypic and genotypic analysis of <i>Candida albicans</i> vaginal isolates reveals that <i>ECE1</i> expression underpins pathogenicity.

    Infection and immunity·2026
    Same journal

    <i>Staphylococcus aureus</i> pore-forming toxins differentially shape disease severity in experimental endophthalmitis.

    Infection and immunity·2026
    Same journal

    Group B streptococcal membrane vesicles induce proinflammatory responses in neonatal meninges.

    Infection and immunity·2026
    Same journal

    <i>Pseudomonas aeruginosa</i> infection causes lysosomal dysfunction in the cystic fibrosis bronchial epithelium.

    Infection and immunity·2026
    Same journal

    The role of probiotics in restoring and maintaining vaginal microbiome health: a review.

    Infection and immunity·2026
    See all related articles

    Streptococcus pneumoniae strains activate the alternative complement pathway via non-immune immunoglobulin G (IgG) binding. This suggests non-specific IgG on the bacterial surface enhances complement activation.

    Area of Science:

    • Immunology
    • Microbiology
    • Complement System

    Background:

    • Streptococcus pneumoniae is a significant human pathogen.
    • The complement system is crucial for innate immunity.
    • Understanding bacterial evasion mechanisms is vital for therapeutic development.

    Purpose of the Study:

    • To investigate the interaction between Streptococcus pneumoniae and the human complement system.
    • To determine the role of Fc-region reactivity of immunoglobulin G (IgG) in complement activation by pneumococci.
    • To differentiate the roles of the classical and alternative complement pathways in pneumococcal complement consumption.

    Main Methods:

    • Studied 62 Streptococcus pneumoniae strains.
    • Blocked classical complement pathway using ethyleneglycol-bios (beta-aminoethyl ether)-N,N-tetraacetic acid and C4 removal.

    Related Experiment Videos

  • Blocked alternative complement pathway via factor B removal.
  • Assessed complement activation and compared with non-immune Fc-region IgG reactivity.

    • Main Results:

    • Classical complement pathway activation was independent of Fc-region IgG reactivity.
    • Highly Fc-reactive pneumococcal strains demonstrated enhanced alternative pathway activation.
    • Alternative pathway activation by pneumococci requires non-immunospecific IgG.

    Conclusions:

    • Non-immune binding of IgG to the pneumococcal surface confers complement-activating properties.
    • This interaction suggests a mechanism for pneumococcal complement evasion or modulation.
    • Findings highlight the importance of IgG-mediated complement activation in pneumococcal pathogenesis.