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Validation of five-colony pool analysis using multiplex real-time PCR for detection of diarrheagenic Escherichia coli
F Barletta1, T J Ochoa, L Ecker
1Instituto de Medicina Tropical, Universidad Peruana Cayetano Heredia, Av. Honorio Delgado 340, San Martin de Porras, Lima 33, Perú. francescabarletta@yahoo.es
Abstract:
Five Escherichia coli colonies/patient were studied to evaluate the reliability of a multiplex real-time PCR assay for detection of diarrheagenic Escherichia coli groups, using a pool of five colonies rather than individual colonies. Sensitivity and specificity were 98% and 100%, respectively, at a fifth of the cost of the individual colony analysis.
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