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Related Concept Videos

Protein Networks02:26

Protein Networks

An organism can have thousands of different proteins, and these proteins must cooperate to ensure the health of an organism. Proteins bind to other proteins and form complexes to carry out their functions. Many proteins interact with multiple other proteins creating a complex network of protein interactions.
These interactions can be represented through maps depicting protein-protein interaction networks, represented as nodes and edges. Nodes are circles that are representative of a protein,...
Protein Networks02:26

Protein Networks

An organism can have thousands of different proteins, and these proteins must cooperate to ensure the health of an organism. Proteins bind to other proteins and form complexes to carry out their functions. Many proteins interact with multiple other proteins creating a complex network of protein interactions.
These interactions can be represented through maps depicting protein-protein interaction networks, represented as nodes and edges. Nodes are circles that are representative of a protein,...
Protein-protein Interfaces02:04

Protein-protein Interfaces

Many proteins form complexes to carry out their functions, making protein-protein interactions (PPIs) essential for an organism's survival. Most PPIs are stabilized by numerous weak noncovalent chemical forces. The physical shape of the interfaces determines the way two proteins interact. Many globular proteins have closely-matching shapes on their surfaces, which form a large number of weak bonds. Additionally, many PPIs occur between two helices or between a surface cleft and a polypeptide...
Protein-Protein Interfaces02:04

Protein-Protein Interfaces

Many proteins form complexes to carry out their functions, making protein-protein interactions (PPIs) essential for an organism's survival. Most PPIs are stabilized by numerous weak noncovalent chemical forces. The physical shape of the interfaces determines the way two proteins interact. Many globular proteins have closely-matching shapes on their surfaces, which form a large number of weak bonds. Additionally, many PPIs occur between two helices or between a surface cleft and a polypeptide...
General Transcription Factors01:30

General Transcription Factors

Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...
Assembly of Signaling Complexes01:30

Assembly of Signaling Complexes

Multiprotein signaling complexes are formed in a dynamic process involving protein-protein interactions at the cytoplasmic domain of transmembrane receptors or enzymatic and non-enzymatic proteins associated with the receptor. These complexes ensure the activation and propagation of intracellular signals that regulate cell functions.
Interaction domains in cell signaling
Interaction domains recognize exposed features of their binding partners containing post-translationally modified sequences,...

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Updated: Jun 24, 2026

Quantification of Protein Interaction Network Dynamics using Multiplexed Co-Immunoprecipitation
07:57

Quantification of Protein Interaction Network Dynamics using Multiplexed Co-Immunoprecipitation

Published on: August 21, 2019

Tissue specificity and the human protein interaction network.

Alice Bossi1, Ben Lehner

  • 1EMBL-CRG Systems Biology Unit, Centre for Genomic Regulation, UPF, Barcelona, Spain.

Molecular Systems Biology
|April 10, 2009
PubMed
Summary
This summary is machine-generated.

Most proteins, even housekeeping ones, engage in tissue-specific interactions. This reveals how specialized cell functions evolve and highlights the dynamic nature of protein networks in humans.

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08:38

Genome-wide Protein-protein Interaction Screening by Protein-fragment Complementation Assay (PCA) in Living Cells

Published on: March 3, 2015

Area of Science:

  • Molecular Biology
  • Systems Biology
  • Genomics

Background:

  • Protein interaction networks are crucial for cellular function.
  • Cellular and tissue-specific protein expression limits potential interactions.
  • Understanding the interplay between expression and interaction is key to deciphering biological complexity.

Purpose of the Study:

  • To analyze the relationship between protein expression and physical interactions in humans.
  • To investigate how tissue-specific expression influences protein interaction networks.
  • To propose a model for the evolution of tissue-specific biology.

Main Methods:

  • Integration of protein interaction data with human protein expression data.
  • Analysis of protein interaction patterns across different cell types and tissues.
  • Characterization of 'housekeeping' versus tissue-specific proteins and their interaction partners.

Main Results:

  • Proteins with restricted cell-type expression, including recently evolved proteins, tend to have fewer physical interactions.
  • Tissue-specific proteins frequently interact with universally expressed proteins, enabling modulation of core cellular processes.
  • Housekeeping proteins, expressed ubiquitously, exhibit a significant number of tissue-specific interactions.

Conclusions:

  • Most proteins, including housekeeping ones, participate in tissue-specific molecular interactions.
  • This finding suggests a model where tissue specificity arises from specialized interactions of broadly expressed proteins.
  • The study redefines our understanding of protein function in the context of tissue-specific biology.