Related Experiment Video
Updated: Jun 24, 2026

Isolation of Fidelity Variants of RNA Viruses and Characterization of Virus Mutation Frequency
Published on: June 16, 2011
Changes in Borna disease virus genome with adaptation to host
Satoshi Okayama1, Nanako Miura, Masaru Murakami
1Laboratory of Veterinary Immunology, Azabu University School of Veterinary Medicine, Fuchinobe, Sagamihara, Japan.
Abstract:
The CRNP5 variant of Borna disease virus (BDV) has stronger pathogenesis than the CRP3 variant in which only 4 nucleotides in the whole genome are different. The CRP3 is produced by 3 passages in rat brains of BDV, whereas the CRNP5 is produced by 5 passages in mouse brains after 2 passages in rat brains of the BDV. Thymidylic acids at nt 3608 and 3673 were replaced by cytidylic acids during 3 passages in mice. Three passages in mice caused replacement of adenylic acid at nt 7936 by guanylic acid. No replacement at nt 8742 occurred during passages in mice.
More Related Videos
Related Concept Videos
Viral Mutations
Size and Structure of Viral Genomes
Viruses with RNA Genomes
Retrovirus Life Cycles
Retroviruses
Viral Recombination

