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Related Experiment Videos

Cutting and closing without recombination in V(D)J joining.

S M Lewis1, J E Hesse

  • 1Division of Biology, California Institute of Technology, Pasadena 91125.

The EMBO Journal
|December 1, 1991
PubMed
Summary
This summary is machine-generated.

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Open and shut junctions, rare V(D)J recombination products, can occur independently of two signals or through interaction with cryptic signals. This mechanism may prevent harmful chromosomal rearrangements in lymphoid tissues.

Area of Science:

  • Molecular Biology
  • Immunology
  • Genetics

Background:

  • V(D)J recombination is crucial for adaptive immunity, generating antibody and T-cell receptor diversity.
  • Open and shut junctions represent an aberrant outcome where DNA cleavage and re-ligation occur without complete recombination.
  • Understanding the mechanisms underlying open and shut junctions is vital for preventing potentially deleterious chromosomal rearrangements.

Purpose of the Study:

  • To investigate the mechanistic origins of open and shut junctions in V(D)J recombination.
  • To determine the role of single versus dual joining signals in the formation of open and shut events.
  • To explore how signal proximity and cryptic signal interactions influence these aberrant outcomes.

Main Methods:

  • In vivo assays using engineered DNA substrates with one or two joining signals.

Related Experiment Videos

  • Analysis of open and shut junction formation in response to varying substrate configurations.
  • Comparative analysis of single-signal and dual-signal substrate outcomes.

    • Main Results:

    • Open and shut events are frequently observed with single consensus joining signals, indicating they do not always require two signals.
    • Dual open and shut events can occur, suggesting a two-signal transaction is sometimes involved.
    • These findings reveal two distinct pathways leading to open and shut junctions.

    Conclusions:

    • Open and shut junctions arise from at least two distinct mechanisms: one independent of signal interaction, and another involving cryptic signal elements.
    • These alternative pathways may serve as a safeguard against detrimental chromosomal rearrangements during V(D)J recombination.
    • The V(D)J recombination machinery's target recognition errors can lead to non-recombinant outcomes, preventing genomic instability.