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Related Concept Videos

Capillary Electrophoresis: Applications01:30

Capillary Electrophoresis: Applications

Capillary electrophoretic separations offer various modes, each with unique applications. These modes include capillary zone electrophoresis, capillary gel electrophoresis, capillary array electrophoresis, capillary isoelectric focusing, capillary isotachophoresis, micellar electrokinetic chromatography, and capillary electrochromatography.
Capillary zone electrophoresis (CZE) separates ionic components based on their electrophoretic mobility. It has been used to separate proteins, amino acids,...
Electrophoresis: Overview01:20

Electrophoresis: Overview

Electrophoresis is a powerful analytical separation technique that relies on the differential migration of charged species when subjected to an electric field. The core strength of electrophoresis lies in its ability to separate high-molecular-weight species in complex mixtures. It has found widespread use in biochemistry, molecular biology, and analytical chemistry, allowing the separation of compounds like amino acids, nucleotides, carbohydrates, and proteins with excellent resolution.
There...
Ion-Exchange Chromatography01:09

Ion-Exchange Chromatography

Ion-exchange chromatography, or IEC, is a technique for separating ions based on their affinity for the stationary phase. The stationary phase is a cross-linked polymer resin with covalently attached ionic functional groups. The functional groups can be either positively charged (cation exchangers) or negatively charged (anion exchangers). A cation exchanger consists of a polymeric anion and active cations, while an anion exchanger is a polymeric cation with active anions. The choice of...
Capillary Electrophoresis: Instrumentation01:20

Capillary Electrophoresis: Instrumentation

Capillary electrophoresis instrumentation typically consists of several key components. A high-voltage power supply generates the electric field necessary for the separation by connecting to an anode (the positively charged electrode) and a cathode (the negatively charged electrode) located in buffer reservoirs at each end of the capillary tube. The system includes a sample vial, a fused silica capillary tube coated with polyimide for mechanical strength through which the sample components...
High-Performance Liquid Chromatography: Types of Detectors01:15

High-Performance Liquid Chromatography: Types of Detectors

The role of the detectors in High-Performance Liquid Chromatography (HPLC) is to analyze the solutes as they exit from the chromatographic column. The detector recognizes the solute's property and generates corresponding electrical signals, which are converted into a readable graph of the detector's response versus elution time called a chromatogram at the computer. There are several types of HPLC detectors, each with its own advantages and limitations, depending on the analyte properties and...
Mass Spectrometry: Complex Analysis01:21

Mass Spectrometry: Complex Analysis

Mass spectrometry is an important technique for the identification of pure compounds. However, it has some limitations for the analysis of complex mixtures, often due to excessive fragmentation making the spectrum too complicated to decipher. Mass spectrometry can be combined with suitable separation methods in sequence, forming hyphenated methods, which are useful in the analysis of complex mixtures.
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...

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Updated: Jun 24, 2026

Capillary Electrophoresis Mass Spectrometry Approaches for Characterization of the Protein and Metabolite Corona Acquired by Nanomaterials
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Capillary Electrophoresis Mass Spectrometry Approaches for Characterization of the Protein and Metabolite Corona Acquired by Nanomaterials

Published on: October 27, 2020

Ecstasy analysis by monolithic materials-capillary electrochromatography.

Aree Choodum1, Panote Thavarungkul, Proespichaya Kanatharana

  • 1Trace Analysis and Biosensor Research Center, Department of Chemistry, Prince of Songkla University, Hat Yai, Songkhla 90112, Thailand.

Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry
|April 11, 2009
PubMed
Summary
This summary is machine-generated.

A new capillary electrochromatography method using a butyl methacrylate monolith column effectively analyzes 3,4-methylenedioxymethamphetamine (MDMA) in ecstasy and its metabolites in urine. This method offers high efficiency and sensitivity for drug analysis.

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High-throughput and Comprehensive Drug Surveillance Using Multisegment Injection-Capillary Electrophoresis-Mass Spectrometry
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High-throughput and Comprehensive Drug Surveillance Using Multisegment Injection-Capillary Electrophoresis-Mass Spectrometry

Published on: April 23, 2019

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Capillary Electrophoresis Mass Spectrometry Approaches for Characterization of the Protein and Metabolite Corona Acquired by Nanomaterials
07:54

Capillary Electrophoresis Mass Spectrometry Approaches for Characterization of the Protein and Metabolite Corona Acquired by Nanomaterials

Published on: October 27, 2020

High-throughput and Comprehensive Drug Surveillance Using Multisegment Injection-Capillary Electrophoresis-Mass Spectrometry
10:17

High-throughput and Comprehensive Drug Surveillance Using Multisegment Injection-Capillary Electrophoresis-Mass Spectrometry

Published on: April 23, 2019

Area of Science:

  • Analytical Chemistry
  • Forensic Science
  • Chromatography

Background:

  • Capillary electrochromatography (CEC) is a powerful separation technique.
  • Analysis of illicit substances like 3,4-methylenedioxymethamphetamine (MDMA) and its metabolites is crucial for forensic toxicology.
  • Development of sensitive and efficient analytical methods is essential for accurate drug detection.

Purpose of the Study:

  • To develop and validate a CEC method for the analysis of MDMA in ecstasy tablets and its metabolites in urine samples.
  • To evaluate the performance of different monolithic columns for this analysis.
  • To determine the efficiency, sensitivity, and precision of the developed method.

Main Methods:

  • Preparation and utilization of monolithic columns (cationic, neutral, anionic) in CEC.
  • Development of a CEC method using a neutral butyl methacrylate (BMA) monolith column.
  • Sample preparation involving ultrasonic extraction for tablets and liquid-liquid extraction for urine.
  • Quantification of MDMA and its metabolites using the developed CEC method.

Main Results:

  • Neutral BMA monolith columns demonstrated superior efficiency, resolution, and peak symmetry compared to anionic and cationic columns.
  • The developed BMA-CEC method achieved detection limits of 1 microg/mL for MDMA and its metabolites.
  • Excellent linearity (R(2) > 0.99) and precision were observed for the method.
  • MDMA was identified as the primary component in ecstasy tablets, and 4-hydroxy-3-methoxymethamphetamine was the major metabolite in urine.
  • High recovery rates were obtained for both MDMA from tablets (97%) and its metabolites from urine (84-102%).

Conclusions:

  • The developed CEC method utilizing a BMA monolithic column is highly efficient and sensitive for the analysis of MDMA and its metabolites.
  • This method provides a reliable tool for the forensic analysis of ecstasy and the monitoring of MDMA metabolism.
  • The BMA monolith column offers significant advantages in terms of chromatographic performance for drug analysis.