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Updated: Jun 24, 2026

Quantitative Analysis of the Cellular Lipidome of Saccharomyces Cerevisiae Using Liquid Chromatography Coupled with Tandem Mass Spectrometry
Published on: March 8, 2020
Tryptic digest analysis by comprehensive reversed phasextwo reversed phase liquid chromatography (RP-LCx2RP-LC) at
Isabelle François1, Deirdre Cabooter, Koen Sandra
1Separation Sciences Group and Pfizer Analytical Research Centre, Ghent University, Ghent, Belgium.
Abstract:
As an alternative to the classical approach of combining strong cation exchange SCX-LC and RP-LC for the separation of complex proteomic samples, this essay describes the online comprehensive RP-LCxRP-LC separation of BSA and human blood serum. High orthogonality and peak capacity are achieved through the application of a significantly different pH in the two dimensions. The coupling of fused-core columns in series ensures high efficiency in the first dimension, while a previously designed interface with parallel second dimension columns further enhances the separation capability of the comprehensive system.
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