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Related Experiment Video

Updated: Jun 24, 2026

Dissolution Dynamic Nuclear Polarization Instrumentation for Real-time Enzymatic Reaction Rate Measurements by NMR

Dissolution Dynamic Nuclear Polarization Instrumentation for Real-time Enzymatic Reaction Rate Measurements by NMR

Published on: February 23, 2016

High-field dynamic nuclear polarization in aqueous solutions.

M J Prandolini¹, V P Denysenkov, M Gafurov

¹Institute of Physical and Theoretical Chemistry and Center for Biomolecular Magnetic Resonance, Goethe-University Frankfurt, 60438 Frankfurt am Main, Germany.

Journal of the American Chemical Society

|April 14, 2009

Summary

High dynamic nuclear polarization (DNP) enhancements over 10 were achieved in liquid water samples at room temperature. This breakthrough in Nuclear Magnetic Resonance (NMR) sensitivity is crucial for studying biomolecules.

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Area of Science:

Magnetic Resonance Spectroscopy
Biophysical Chemistry

Background:

Dynamic Nuclear Polarization (DNP) enhances Nuclear Magnetic Resonance (NMR) sensitivity.
High-field NMR requires sensitive techniques for analyzing biomolecules at low concentrations.

Purpose of the Study:

To achieve significant DNP enhancements in liquid water samples at room temperature.
To demonstrate the feasibility of DNP for high-resolution NMR applications.

Main Methods:

Utilized a double-resonance structure for in situ polarization of liquid samples.
Simultaneous excitation of NMR and Electron Paramagnetic Resonance (EPR) transitions.
Employed low incident microwave power (45 mW).

Main Results:

Achieved DNP enhancements greater than 10 in liquid water.
Demonstrated high sensitivity at 9.2 T (400 MHz 1H NMR, 260 GHz EPR).
Successful polarization at very low microwave power.

Conclusions:

This study represents a significant advancement in DNP technology.
The findings pave the way for DNP applications in high-resolution NMR.
Enhanced sensitivity is critical for analyzing biomolecules in small volumes and at physiological concentrations.