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Multiplexed sandwich immunoassays using electrochemiluminescence imaging resolved at the single bead level

Frédérique Deiss1, Christopher N LaFratta, Matthew Symer

  • 1Universite Bordeaux, Institut des Sciences Moleculaires, ENSCPB, 16 Avenue Pey-Berland, 33607 Pessac, France.

Journal of the American Chemical Society
|April 14, 2009
PubMed

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Enzyme-Linked Immunosorbent Assay01:33

Enzyme-Linked Immunosorbent Assay

In 1971, Peter Perlman and Eva Engvall developed an Enzyme-linked immunosorbent assay (ELISA or EIA). ELISA differs from western blot in that the assays are conducted in microtiter plates or in vivo rather than on an absorbent membrane.
There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen.  Enzyme-substrate reaction allows the antigen to be visualized or quantified.