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Updated: Jun 24, 2026

Monitoring Endoplasmic Reticulum Calcium Homeostasis Using a Gaussia Luciferase SERCaMP
Published on: September 6, 2015
[Endoplasmic reticulum stress: light my fIRE]
Marion Bouchecareilh1, Eric Chevet
1Avenir Inserm U889, Université Bordeaux 2, 146, rue Léo Saignat, 33076 Bordeaux, France.
Abstract:
Upon accumulation of misfolded proteins in the lumen of the endoplasmic reticulum (ER), a specific adaptive response, named the Unfolded Protein Response (UPR) is activated in order to protect cells from this stress. In metazoans, the UPR is mediated by three transmembrane ER resident proteins: PERK, ATF6 and IRE1. Among these, only IRE1 is found to be conserved from yeasts to mammals. IRE1 is a type I transmembrane protein which bears two enzymatic activities serine/threonine kinase and endoribonuclease. Crystal structures of S. cerevisiae luminal and cytosolic domains allowed a better understanding of its activation mode. However, IRE1 regulatory mechanisms and IRE1-dependent signalling pathways still remain to be fully explored. This review will present current knowledge on IRE1 protein and focus on its roles in physiological and pathophysiological processes.
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