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A sensitive impedimetric thrombin aptasensor based on polyamidoamine dendrimer.

Zhanxia Zhang1, Wen Yang, Juan Wang

  • 1State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Renmin Street 5625, Changchun, Jilin 130022, China.

Talanta
|April 14, 2009
PubMed
Summary

This study presents a novel aptasensor for detecting thrombin using a polyamidoamine dendrimer modified gold electrode. The developed impedimetric aptasensor offers high sensitivity and a low detection limit for thrombin determination in biological fluids.

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Area of Science:

  • Electrochemistry
  • Biosensors
  • Nanotechnology

Background:

  • Thrombin is a key biomarker in hemostasis and thrombosis.
  • Accurate and sensitive detection of thrombin is crucial for diagnosing and monitoring coagulation disorders.
  • Existing detection methods often require complex procedures or lack sufficient sensitivity.

Purpose of the Study:

  • To develop a label-free and highly sensitive impedimetric aptasensor for thrombin detection.
  • To utilize polyamidoamine dendrimer and aptamer immobilization on a gold electrode.
  • To evaluate the performance of the aptasensor in terms of sensitivity, selectivity, stability, and applicability in biological fluids.

Main Methods:

  • Fabrication of a gold electrode modified with polyamidoamine dendrimer and thrombin aptamer probe.

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  • Utilizing glutaraldehyde for covalent attachment and activation.
  • Employing electrochemical impedance spectroscopy (EIS) for signal transduction.
  • Surface plasmon resonance (SPR) for monitoring assembly process.
  • Detection of thrombin using the [Fe(CN)6](3-/4-) redox couple.

    • Main Results:

    • A linear relationship was observed between charge-transfer resistance (Rct) and thrombin concentration (1-50 nM).
    • A low detection limit of 0.01 nM (S/N=3) was achieved.
    • The aptasensor demonstrated good regeneration ability, selectivity, and stability.
    • Successful detection of thrombin in biological fluid was confirmed.

    Conclusions:

    • The developed polyamidoamine dendrimer-based impedimetric aptasensor offers a sensitive and label-free platform for thrombin detection.
    • Covalent immobilization strategies enhanced probe immobilization and signal amplification.
    • The aptasensor shows potential for clinical applications in thrombin monitoring.