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Multiplex STR typing of aortic tissues from unidentified cadavers
Yayoi Sato1, Hisako Motani, Hiroyuki Inoue
1Department of Legal Medicine, Graduate School of Medicine, Chiba University, 1-8-1, Inohana, Chuo-ku, Chiba City, Chiba 260-8670, Japan. yappe@faculty.chiba-u.jp
Legal Medicine (Tokyo, Japan)
|April 14, 2009
Summary
Aortic tissue DNA is effective for identifying unidentified bodies. Multiplex short tandem repeat (STR) typing can successfully identify individuals up to one month post-mortem, aiding forensic investigations.
Area of Science:
- Forensic Science
- Genetics
- Molecular Biology
Background:
- Accurate identification of unidentified bodies is crucial in forensic investigations.
- Autopsy-derived tissues offer potential sources for DNA analysis.
- Short tandem repeat (STR) typing is a standard method for human identification.
Purpose of the Study:
- To evaluate the efficacy of aortic tissue DNA for forensic identification.
- To determine the post-mortem interval (PMI) suitability of aortic tissue for STR typing.
Main Methods:
- DNA extraction from aortic tissues of 47 unidentified cadavers.
- Multiplex short tandem repeat (STR) typing using the AmpFlSTR Identifiler kit.
- Analysis of DNA quality and quantity, and STR allele detection.
Main Results:
- Successful STR typing achieved in 98% of samples.
- Complete STR and amelogenin profiling obtained in 81% of cases.
- Typing success correlated with post-mortem interval, with optimal results within 1 month.
Conclusions:
- Aortic tissue is a valuable source for DNA-based identification of unidentified bodies.
- Multiplex STR analysis of aortic DNA is reliable for forensic identification up to approximately one month post-mortem.
- Degradation of longer DNA fragments increases with post-mortem time, impacting typing success.
