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Sitosterol bioconversion with resting cells in liquid polymer based systems.

Filipe Carvalho¹, Marco P C Marques, Carla C C R de Carvalho

¹IBB-Institute for Biotechnology and Bioengineering, Centre for Biological and Chemical Engineering, Instituto Superior Técnico, Lisboa, Portugal.

Bioresource Technology

|April 14, 2009

Summary

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Liquid polymers, like silicone B oil, offer a greener alternative for microbial bioconversions of hydrophobic compounds, improving yields in multi-step processes.

Area of Science:

Biotechnology
Industrial Microbiology
Green Chemistry

Background:

Aqueous bioconversion systems struggle with low volumetric productivity for hydrophobic compounds.
Increasing environmental regulations restrict the use of traditional organic solvents in industrial bioprocesses.
Alternative bioconversion media, including supercritical fluids, ionic liquids, and natural oils, are under investigation.

Purpose of the Study:

To evaluate liquid polymers as a sustainable component in bioconversion media.
To assess the efficacy of silicone B oil in a multi-step microbial bioconversion process.
To optimize bioconversion yields for hydrophobic compounds in an environmentally friendly system.

Main Methods:

Utilized a model system: selective side-chain cleavage of beta-sitosterol.

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Employed free resting cells of Mycobacterium sp. NRRL B-3805.

Investigated silicone B oil as a substrate carrier and product pool in single and two-phase (oil:buffer) systems.

Main Results:

High product yields were achieved using silicone B oil as the bioconversion medium.
The silicone B oil effectively served as both substrate carrier and product pool.
Both single-phase (oil) and two-phase (oil:buffer) systems demonstrated successful outcomes.

Conclusions:

Liquid polymers, specifically silicone B oil, are effective in enhancing bioconversion yields for hydrophobic compounds.
Silicone B oil presents a viable, environmentally friendlier alternative to traditional organic solvents.
This approach supports the development of sustainable industrial bioprocesses for complex metabolic pathways.