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Updated: Jun 24, 2026

Photoconversion of Purified Fluorescent Proteins and Dual-probe Optical Highlighting in Live Cells
Published on: June 26, 2010
Photoconversion in orange and red fluorescent proteins
Gert-Jan Kremers1, Kristin L Hazelwood, Christopher S Murphy
1Department of Molecular Physiology and Biophysics, Vanderbilt University, Nashville, Tennessee, USA.
Abstract:
We found that photoconversion is fairly common among orange and red fluorescent proteins, as in a screen of 12 proteins, 8 exhibited photoconversion. Specifically, three red fluorescent proteins could be switched to a green state, and two orange variants could be photoconverted to a far-red state. The orange proteins are ideal for dual-probe highlighter applications, and they exhibited the most red-shifted excitation of all fluorescent proteins described to date.
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