Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Cells and Secretions of the Pancreas01:16

Cells and Secretions of the Pancreas

The pancreas, a vital organ within the abdominal cavity, plays dual roles in the digestive and endocrine systems, collaborating with exocrine and endocrine cells to maintain optimal digestion and blood sugar levels.
Exocrine function is carried out by acinar cells, organized into clusters known as acini. These cells contribute to digestion by releasing substantial quantities of enzyme-rich, alkaline digestive juices.
Concurrently, the dispersed clusters of endocrine cells throughout the...
Glucose Homeostasis: Pancreatic Islets and Insulin Secretion01:27

Glucose Homeostasis: Pancreatic Islets and Insulin Secretion

The pancreatic islets comprising only 1%-2% of the volume are highly vascularized and innervated mini-organs. They contain five endocrine cell types, including β cells that secrete insulin, which is synthesized as a single polypeptide chain, preproinsulin, processed to proinsulin, and finally to insulin and C-peptide. This process is complex and regulated, involving the Golgi complex, the endoplasmic reticulum, and the secretory granules of the β cell.
Insulin and C-peptide are co-secreted in...
Insulin Secretory Vesicles01:05

Insulin Secretory Vesicles

Insulin secretory vesicles release insulin to stimulate blood glucose uptake and regulate carbohydrate metabolism. When the blood glucose levels increase, glucose enters the pancreatic β-islet cells through glucose transporters. Once inside, glucose is metabolized through glycolysis, the citric acid cycle, and the electron transport chain, producing ATP. This increase in ATP concentration closes ATP-sensitive potassium channels, leading to depolarization of the membrane and the opening of...
Pancreas01:19

Pancreas

The pancreas, an essential organ in the human body, is a pinkish-gray elongated structure located posterior to the stomach. It extends laterally from the duodenum towards the spleen and is firmly bound to the posterior wall of the abdominal cavity. The organ's surface has a lumpy, lobular texture that gives it a unique appearance.
The broad head of the pancreas lies within the loop formed by the duodenum, while its slender body reaches towards the spleen. The tail of the pancreas is short and...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Ironing Out Possible Micronutrient Deficiencies Associated with Incretin Receptor Agonist-Based Therapies: Proposed Practical Strategies to Prevent and Manage Iron Deficiency.

Nutrients·2026
Same author

Corrigendum to Correction of diabetes mellitus by transplanting minimal mass of syngeneic islets into vascularized small intestinal segment American Journal of Transplantation. Volume 13, Issue 10, October 2013, Pages 2550-2557.

American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons·2026
Same author

Ceramide metabolism in oxidative and glycolytic muscle: Significance for lipid-induced insulin resistance.

Molecular metabolism·2026
Same author

Optimizing 3D-printed Scaffold Geometry Decreases Foreign Body Response and Enhances Allogeneic Islet Transplant Outcomes.

bioRxiv : the preprint server for biology·2026
Same author

Pancreatic Islet Transplantation in Extrahepatic Sites: Evaluation of the Venous Sac in Large Mammal Models.

Diabetes & metabolism journal·2025
Same author

Combined Use of Vitamin D and DPP-4 Inhibitors as a Potential Adjuvant Treatment Strategy to Enhance the Efficacy of Novel Beta-Cell Replacement Therapies for Type 1 Diabetes.

Medical sciences (Basel, Switzerland)·2025

Related Experiment Video

Updated: Jun 24, 2026

Single-cell Transcriptomic Analyses of Mouse Pancreatic Endocrine Cells
10:05

Single-cell Transcriptomic Analyses of Mouse Pancreatic Endocrine Cells

Published on: September 30, 2018

Endocrine cell clustering during human pancreas development.

Jongmin Jeon1, Mayrin Correa-Medina, Camillo Ricordi

  • 1Diabetes Research Institute, University of Miami Leonard M. Miller School of Medicine, 1450 NW 10th Avenue (R-134), Miami, FL 33136, USA.

The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society
|April 15, 2009
PubMed
Summary

Understanding human pancreas development is key for creating insulin-producing cells from stem cells. This study maps key factors during fetal development, aiding in vitro differentiation protocols.

More Related Videos

Isolation, Culture, and Imaging of Human Fetal Pancreatic Cell Clusters
08:09

Isolation, Culture, and Imaging of Human Fetal Pancreatic Cell Clusters

Published on: May 18, 2014

Generation of Scaffold-free, Three-dimensional Insulin Expressing Pancreatoids from Mouse Pancreatic Progenitors In Vitro
09:33

Generation of Scaffold-free, Three-dimensional Insulin Expressing Pancreatoids from Mouse Pancreatic Progenitors In Vitro

Published on: June 2, 2018

Related Experiment Videos

Last Updated: Jun 24, 2026

Single-cell Transcriptomic Analyses of Mouse Pancreatic Endocrine Cells
10:05

Single-cell Transcriptomic Analyses of Mouse Pancreatic Endocrine Cells

Published on: September 30, 2018

Isolation, Culture, and Imaging of Human Fetal Pancreatic Cell Clusters
08:09

Isolation, Culture, and Imaging of Human Fetal Pancreatic Cell Clusters

Published on: May 18, 2014

Generation of Scaffold-free, Three-dimensional Insulin Expressing Pancreatoids from Mouse Pancreatic Progenitors In Vitro
09:33

Generation of Scaffold-free, Three-dimensional Insulin Expressing Pancreatoids from Mouse Pancreatic Progenitors In Vitro

Published on: June 2, 2018

Area of Science:

  • Developmental Biology
  • Endocrinology
  • Stem Cell Biology

Background:

  • Efficient differentiation of human embryonic stem cells (hESCs) into insulin-producing beta cells requires understanding human endocrine cell generation.
  • Knowledge of spatiotemporal expression profiles of instructive factors is crucial for developing reproducible protocols.

Purpose of the Study:

  • To characterize islet formation and expression profiles of key factors during human pancreas development (7-21 weeks gestational age).
  • To provide data beneficial for guided in vitro differentiation of hES cells into insulin-producing cells.

Main Methods:

  • Human fetal pancreases (7-21 weeks gestational age) were collected.
  • Immunostaining, in situ hybridization, and real-time RT-PCR expression analyses were performed.

Main Results:

  • Islet-like structures emerged around week 12.
  • Fetal islets initially showed aggregated insulin- or glucagon-expressing cells, unlike adult islets.
  • The study period (7-22 weeks) showed decreased progenitor cell proliferation, increased differentiation, NGN3 expression initiation, and appearance of endocrine cells.

Conclusions:

  • Detailed characterization of human pancreas development provides insights into islet formation.
  • Expression profiles of key intrinsic and extrinsic factors were identified.
  • This information supports the development of efficient hESC differentiation protocols for beta cell generation.