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Related Experiment Videos

G protein modulation by estrogens.

C Bouvier1, G Lagacé, R Collu

  • 1Research Unit on Reproductive and Developmental Biology, Ste-Justine Hospital, Montreal, Quebec, Canada.

Molecular and Cellular Endocrinology
|August 1, 1991
PubMed
Summary
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Ovarian hormones, especially estrogens, significantly alter pituitary G protein levels in rats. This hormonal influence, particularly on alpha s42, is linked to changes in prolactin (PRL) secretion.

Area of Science:

  • Endocrinology
  • Neuroscience
  • Molecular Biology

Background:

  • G protein subunits are crucial signaling molecules in the anterior pituitary and striatum.
  • Ovarian hormones, including estrogen, play a significant role in regulating reproductive functions and hormone secretion.
  • Previous research suggests a link between G proteins and hormone regulation, but specific effects of ovarian hormones on pituitary G proteins are not fully understood.

Purpose of the Study:

  • To investigate the impact of ovarian hormone modifications on G protein subunit levels in rat anterior pituitary and striatum.
  • To determine the correlation between G protein levels and plasma prolactin (PRL) levels under varying hormonal conditions.
  • To explore sex-based differences in G protein expression in these tissues.

Main Methods:

Related Experiment Videos

  • Assay of G protein subunit levels using immunoblot and densitometry with specific antibodies.
  • Treatment of ovariectomized rats with 17 beta-estradiol to modify hormone levels.
  • Comparison of G protein levels between female rats (varying estrous cycle phases and hormone treatments) and male rats.

Main Results:

  • Estrogen treatment in ovariectomized rats time-dependently increased plasma PRL levels.
  • Estrogen significantly reduced pituitary G protein levels (except alpha i1 and alpha s42), with alpha s42 increasing and correlating with PRL levels.
  • Pituitary G protein levels varied during the estrous cycle and showed sex differences (lower in females than males), while striatal G proteins remained unaffected.

Conclusions:

  • Ovarian hormones, particularly estrogens, specifically modulate pituitary G protein levels.
  • These changes in pituitary G proteins likely influence the secretion of hormones such as prolactin (PRL).
  • G protein expression in the striatum is not significantly affected by ovarian hormones or sex differences.