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Related Concept Videos

Stem Cell Culture01:17

Stem Cell Culture

Stem cell research aims to find ways to use stem cells to regenerate and repair cellular damage. Over time, most adult cells undergo the wear and tear of aging and lose their ability to divide and repair themselves. Stem cells do not display a particular morphology or function. Adult stem cells, which exist as a small subset of cells in most tissues, keep dividing and can differentiate into a number of specialized cells generally formed by that tissue. These cells enable the body to renew and...
Adult Stem Cells01:33

Adult Stem Cells

Stem cells are undifferentiated cells that divide and produce more stem cells or progenitor cells that differentiate into mature, specialized cell types. All the cells in the body are generated from stem cells in the early embryo, but small populations of stem cells are also present in many adult tissues including the bone marrow, brain, skin, and gut. These adult stem cells typically produce the various cell types found in that tissue—to replace cells that are damaged or to continuously renew...
Source And Potency Of Stem Cells01:27

Source And Potency Of Stem Cells

Stem cells are undifferentiated cells with extensive self-renewal properties that help them maintain their population during the fetal and adult stages of life. They can specialize in all cell types of the human body. However, their differential potential may vary and can be classified into five types. Stem cells can be (1) Totipotent, (2) Pluripotent, (3) Multipotent, (4) Oligopotent, and (5) Unipotent. Each stem cell has a specific origin; the fertilized egg or zygote is a totipotent cell and...

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Related Experiment Video

Updated: Jun 24, 2026

Isolation, Culture, and Characterization of Dental Pulp Stem Cells from Human Deciduous and Permanent Teeth
02:33

Isolation, Culture, and Characterization of Dental Pulp Stem Cells from Human Deciduous and Permanent Teeth

Published on: May 17, 2024

Dental pulp stem cells and their characterization.

Jakub Suchanek1, Tomas Soukup, Benjamin Visek

  • 1Department of Dentistry, Charles University in Prague, Faculty of Medicine in Hradec Kralove, Czech Republic. suchanekj@lfhk.cuni.cz

Biomedical Papers of the Medical Faculty of the University Palacky, Olomouc, Czechoslovakia
|April 15, 2009
PubMed
Summary
This summary is machine-generated.

Supplementing cultivation media with ITS significantly boosted dental pulp stem cell (DPSC) proliferation. This enhancement improved cell doubling times without altering DPSC biological properties or phenotype.

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Isolation, Characterization and Comparative Differentiation of Human Dental Pulp Stem Cells Derived from Permanent Teeth by Using Two Different Methods
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Isolation, Characterization and Comparative Differentiation of Human Dental Pulp Stem Cells Derived from Permanent Teeth by Using Two Different Methods

Published on: November 24, 2012

Primary Culture of Dental Pulp Stem Cells
03:45

Primary Culture of Dental Pulp Stem Cells

Published on: May 5, 2023

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Last Updated: Jun 24, 2026

Isolation, Culture, and Characterization of Dental Pulp Stem Cells from Human Deciduous and Permanent Teeth
02:33

Isolation, Culture, and Characterization of Dental Pulp Stem Cells from Human Deciduous and Permanent Teeth

Published on: May 17, 2024

Isolation, Characterization and Comparative Differentiation of Human Dental Pulp Stem Cells Derived from Permanent Teeth by Using Two Different Methods
14:52

Isolation, Characterization and Comparative Differentiation of Human Dental Pulp Stem Cells Derived from Permanent Teeth by Using Two Different Methods

Published on: November 24, 2012

Primary Culture of Dental Pulp Stem Cells
03:45

Primary Culture of Dental Pulp Stem Cells

Published on: May 5, 2023

Area of Science:

  • Stem Cell Biology
  • Tissue Engineering
  • Regenerative Medicine

Background:

  • Dental pulp stem cells (DPSCs) are a valuable source of mesenchymal stem cells.
  • Understanding their cultivation and properties is crucial for regenerative applications.

Purpose of the Study:

  • To isolate and cultivate DPSCs.
  • To investigate the impact of different media supplements on DPSC proliferation and characteristics.
  • To analyze DPSC phenotype and biological properties.

Main Methods:

  • DPSCs isolated from impacted third molars.
  • Cultivation in media with varying fetal calf serum (FCS) concentrations and ITS supplementation.
  • Assessment of cell viability, doubling time, and phenotype via flow cytometry.

Main Results:

  • DPSCs were successfully cultivated over 40 population doublings in all tested media.
  • ITS supplementation significantly reduced population doubling time.
  • High viability (>90%) and characteristic mesenchymal stem cell markers (CD29, CD44, CD90) were maintained.

Conclusions:

  • ITS supplementation is a key factor in enhancing DPSC proliferative activity.
  • Cultivation conditions did not negatively affect DPSC viability or phenotype.
  • Optimized DPSC expansion is achievable for potential therapeutic uses.