Related Experiment Video
Updated: Aug 3, 2026

High Throughput Microinjections of Sea Urchin Zygotes
Published on: January 21, 2014
Characterization of sea urchin egg endoplasmic reticulum in cortical preparations
1Marine Biological Laboratory, Woods Hole, Massachusetts.
Abstract:
The cortical endoplasmic reticulum (ER) of sea urchin eggs was localized on isolated egg cortices by staining with aqueous suspensions of the dicarbocyanine "DiI." Immunofluorescence localization of a calsequestrin-like protein was essentially identical; this is consistent with a role for the ER in calcium regulation. The ER often encircles cortical granules, making it well-suited for initiating fusion and propagating the calcium wave. Thiazole orange and Hoechst dye 33258 at pH 2 stain ribosomes bound to the ER, providing evidence that the cortical ER is rough ER. High chloride concentrations were found to disrupt ER continuity.
Related Concept Videos
Endoplasmic Reticulum
ER Retrieval Pathway
The ER uses many checkpoints to prevent the entry of incorrectly folded or a resident protein as cargo onto a transport vesicle. These mechanisms...
The Endoplasmic Reticulum

