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A Method for Selecting Structure-switching Aptamers Applied to a Colorimetric Gold Nanoparticle Assay
Published on: February 28, 2015
Label free optical sensor for Avidin based on single gold nanoparticles functionalized with aptamers
Frank Jeyson Hernandez1, Srujan Kumar Dondapati, V Cengiz Ozalp
1Department of Chemical Engineering, Universitat Rovira i Virgili, Tarragona, Spain.
Abstract:
Optical spectroscopy of a single gold nanoparticle, functionalized with an aptamer, is used to sense the specific binding of avidin. Herewith, the field of single noble metal nanoparticle biosensors is extended to the important field of aptamer based assays. The sensitivity of this initial, but not yet optimized apta-nano-sensor is in the range of 20 nM. Due to its nanoscopic size, this single nanoparticle based apta-sensor may be used in nanoscopic volumes such as in array type assays or even inside cells.

