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Updated: Jun 24, 2026
Isolating Brown Adipocytes from Murine Interscapular Brown Adipose Tissue for Gene and Protein Expression Analysis
Published on: March 12, 2021
Molecular cloning, gene expression, and tissue distribution of adiponectin and its receptors in the Japanese monkey,
Kyung-Hwa Kang1, Atsunori Higashino, Heui-Soo Kim
1Center for Human Evolution Modeling Research, Primate Research Institute, Kyoto University, Inuyama Aichi, Japan. ghkang@deu.ac.kr
Background:
Adiponectin is an adipocyte-derived hormone that affects regulation of metabolic syndrome such as insulin resistance, type-2 diabetes, and obesity. It functions via seven transmembrane domain receptors [i.e.,adiponectin receptors 1 (AdipoR1) and 2 (AdipoR2)] that have been scarcely investigated in non-human primates.
Methods:
Molecular cloning of cDNAs for adiponectin, AdipoR1, and AdipoR2 that included the whole protein-coding region in the Japanese monkey, Macaca fuscata, was carried out. Tissue-specific expression of respective genes was analyzed with Northern blot hybridization.
Results:
The essential Cys36 and four lysine residues in adiponectin, and transmembrane-spanning domains in AdipoR1 and AdipoR2 appear well conserved. While adiponectin mRNA is expressed only in adipose tissues, AdipoR1 mRNA was found to be expressed in various tissues including the brain.
Conclusions:
These results significantly add to the understanding of the molecular basis of obesity-related adipokines and their receptors in nonhuman primates.
