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Aptamer-based SERRS sensor for thrombin detection.

Hansang Cho1, Brian R Baker, Sebastian Wachsmann-Hogiu

  • 1Biomolecular Nanotechnology Center, Berkeley Sensor & Actuator Center, Department of Bioengineering, University of California, Berkeley, California 94720, USA.

Nano Letters
|April 16, 2009
PubMed
Summary
This summary is machine-generated.

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This study presents a stable aptamer-based SERRS sensor for detecting human alpha-thrombin. The sensor shows high sensitivity and specificity, enabling potential clinical diagnostic applications.

Area of Science:

  • Biomedical Engineering
  • Analytical Chemistry
  • Biotechnology

Background:

  • Accurate detection of coagulation proteins like human alpha-thrombin is crucial for diagnosing and managing bleeding disorders.
  • Existing detection methods may lack the sensitivity, specificity, or stability required for robust clinical diagnostics.

Purpose of the Study:

  • To develop and characterize a novel aptamer-based Surface-Enhanced Resonance Raman Scattering (SERRS) sensor for sensitive and specific detection of human alpha-thrombin.
  • To evaluate the sensor's stability and performance in complex biological matrices for potential clinical applications.

Main Methods:

  • Immobilization of thrombin-binding aptamers on a SERRS-active substrate.
  • Monitoring changes in SERRS signal intensity upon specific binding of human alpha-thrombin to the immobilized aptamers.

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  • Assessment of sensor selectivity using control oligonucleotides and other protein analytes.
  • Testing sensor performance in the presence of biological fluids like fetal calf serum.

    • Main Results:

    • The aptamer-based SERRS sensor achieved a high sensitivity with a limit of detection of 100 pM for human alpha-thrombin.
    • The sensor demonstrated excellent specificity, discriminating thrombin from other proteins.
    • The immobilized aptamer proved robust, enabling detection of 1 nM thrombin in 10% fetal calf serum.
    • The sensor design allows for potential multiplexed detection.

    Conclusions:

    • The developed aptamer-based SERRS sensor offers a highly sensitive, specific, and stable platform for human alpha-thrombin detection.
    • The sensor's robustness in complex biofluids supports its potential for clinical diagnostic applications.
    • The proposed SERRS sensor technology holds promise for advancing coagulation diagnostics and could be extended to multiplexed assays.