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Notch Signaling Pathway

Notch Signaling Pathway

The Notch signaling pathway is a major intracellular signaling pathway that is highly conserved over a broad spectrum of metazoan species. It stands unique from other intracellular signaling mechanisms in animals because notch protein itself acts as the receptor as well as the primary signaling molecule.
The Notch gene came into the limelight in 1914 after the discovery that its mutation in Drosophila melanogaster leads to a serrated (or "notched") wing margin phenotype. It was not until 1985...

Notch Signaling Pathway

Notch Signaling Pathway

The Notch signaling pathway is a major intracellular signaling pathway that is highly conserved over a broad spectrum of metazoan species. It stands unique from other intracellular signaling mechanisms in animals because notch protein itself acts as the receptor as well as the primary signaling molecule.
The Notch gene came into the limelight in 1914 after the discovery that its mutation in Drosophila melanogaster leads to a serrated (or "notched") wing margin phenotype. It was not until 1985...

Role Of Notch Signalling In Intestinal Stem Cell Renewal

Role Of Notch Signalling In Intestinal Stem Cell Renewal

Notch signaling was first discovered in Drosophila melanogaster, where it is involved in cell lineage differentiation. Notch signaling regulates the maintenance and differentiation of intestinal stem cells or ISCs by controlling the expression of atonal homolog 1 or Atoh1. Atoh1 directs cells to differentiate into secretory cells.
Direct cell-to-cell contact is needed for the activation of Notch signaling. The signal is initiated when a notch ligand binds to a receptor on an adjacent cell, also...

Liver Regeneration

Liver Regeneration

The liver is an important organ in vertebrates that plays an essential role in metabolism. It is also responsible for storing and redistributing nutrients such as carbohydrates, fats, and vitamins in the body. Additionally, the liver releases bile salts which are critical for digesting food and eliminating toxic metabolites from the body.
Cells of Liver
The liver comprises four major types of cells— hepatocytes, stellate, Kupffer, and sinusoidal endothelial cells. The hepatocytes are large...

Hedgehog Signaling Pathway

Hedgehog Signaling Pathway

The Hedgehog gene (Hh) was first discovered due to its control of the growth of disorganized, hair-like bristles phenotype in Drosophila, much like hedgehog spines. Hh plays a crucial role in the development of organs and the maintenance of homeostasis in both invertebrates and vertebrates. However, while Drosophila has only one Hh protein, mammals have multiple functional Hedgehog proteins - Sonic (Shh), Desert (Dhh), and Indian Hedgehog (Ihh). All of these homologous proteins have adapted to...

Cell Specific Gene Expression

Cell Specific Gene Expression

Multicellular organisms contain a variety of structurally and functionally distinct cell types, but the DNA in all the cells originated from the same parent cells. The differences in the cells can be attributed to the differential gene expression. Liver cells, whose functions include detoxification of blood, production of bile to metabolize fats, and synthesis of proteins essential for metabolism, must express a specific set of genes to perform their functions. Gene expression also varies with...

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Related Experiment Video

Updated: Jun 24, 2026

Determining Bile Duct Density in the Mouse Liver

Determining Bile Duct Density in the Mouse Liver

Published on: April 30, 2019

Notch signaling controls liver development by regulating biliary differentiation.

Yiwei Zong¹, Archana Panikkar, Jie Xu

¹Abramson Family Cancer Research Institute, University of Pennsylvania School of Medicine, Philadelphia, PA 19104, USA.

Development (Cambridge, England)

|April 17, 2009

Summary

This summary is machine-generated.

Notch signaling drives bile duct formation in the liver by coordinating cell differentiation and tubule development. This pathway reprograms hepatocytes into ductal cells, clarifying its role in liver development.

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Determining Bile Duct Density in the Mouse Liver

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Published on: April 30, 2019

In Vitro Cultivation Techniques for Modeling Liver Organogenesis, Building Assembloids, and Designing Synthetic Tissues using Human Cell Lines

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A High-throughput Cell Microarray Platform for Correlative Analysis of Cell Differentiation and Traction Forces

A High-throughput Cell Microarray Platform for Correlative Analysis of Cell Differentiation and Traction Forces

Published on: March 1, 2017

Area of Science:

Developmental biology
Hepatology
Cell signaling

Background:

Bile transport in mammals relies on a complex bile duct network.
Notch signaling is crucial for bile duct formation, but its precise mechanism remains elusive.

Purpose of the Study:

To elucidate the role and mechanism of Notch signaling in mammalian liver bile duct development.
To investigate how Notch signaling influences biliary fate and morphogenesis.

Main Methods:

Utilized mouse models to study bile duct development.
Investigated Notch signaling activation in liver progenitor cells and hepatocytes.
Examined the effects of Notch pathway activation on biliary differentiation and tubule formation.

Main Results:

Identified a novel tubulogenesis mechanism involving sequential radial differentiation in bile duct formation.
Demonstrated that Notch signaling activation is essential for biliary fate acquisition.
Showed that Notch signaling promotes ectopic biliary differentiation and tubule formation in a dose-dependent manner.
Revealed that Notch signaling can reprogram postnatal hepatocytes to adopt a biliary fate, mimicking normal development.

Conclusions:

Notch signaling coordinates biliary differentiation and morphogenesis during liver development.
The findings reconcile conflicting reports on Notch's role in liver development.
Suggests Notch signaling as a key regulator of bile duct formation and potential therapeutic target.