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Related Concept Videos

Phosphoinositides and PIPs01:42

Phosphoinositides and PIPs

Phosphoinositides are a group of phospholipids containing a glycerol backbone with two fatty acid chains and a phosphate attached to a myoinositol sugar ring. The inositol head group extends into the cytoplasm, where it is modified by adding phosphate groups to form phosphatidylinositol phosphates or PIPs.
Different phosphoinositides are synthesized and recruited on the cytosolic face of the plasma membrane. The localization of specific phosphoinositides concentrated in separate membrane...
Overview of Lipid Metabolism01:24

Overview of Lipid Metabolism

Lipid metabolism is a crucial process in the human body that involves the synthesis and degradation of lipids. This process is essential for energy production, cell membrane formation, and hormone production, among other functions.
Lipolysis: The Breakdown of Lipids:
Lipolysis is the process of breaking down lipids, particularly triglycerides, into glycerol and fatty acids. This process typically occurs in the adipose tissue and is triggered by various hormones, including glucagon and...
IP3/DAG Signaling Pathway01:11

IP3/DAG Signaling Pathway

Membrane lipids such as phosphatidylinositol (PI) are precursors for several membrane-bound and soluble second messengers. Specific kinases phosphorylate PI and produce phosphorylated inositol phospholipids. One such inositol phospholipids are the  phosphatidylinositol-4,5 bisphosphate [PI(4,5)P2], present in the inner half of the lipid bilayer. Upon ligand binding, GPCR stimulates Gq proteins to turn on phospholipase Cꞵ. Activated phospholipase Cꞵ cleaves PI(4,5)P2 and produces two-second...
Lipid Catabolism01:25

Lipid Catabolism

Triglycerides serve as crucial long-term energy storage molecules in microorganisms, providing a dense source of metabolic energy. Their breakdown is mediated by lipases, which hydrolyze triglycerides into glycerol and free fatty acids. Each of these components follows distinct metabolic pathways, ultimately contributing to ATP synthesis and cellular energy homeostasis.Glycerol MetabolismGlycerol, released from triglyceride hydrolysis, is phosphorylated by glycerol kinase to form...
Lipid Digestion01:06

Lipid Digestion

Lipids are large molecules that are generally not water-soluble. Since most of the digestive enzymes in the human body are water-based, there are specific steps the body must take to break down lipids and make them available for use.
GPI Anchoring of Proteins in the ER Membrane01:29

GPI Anchoring of Proteins in the ER Membrane

GPI-anchoring is a post-translational, reversible protein modification that is ubiquitous in eukaryotes. Such proteins are primarily present on the exoplasmic leaflet of the plasma membrane.
GPI-anchor structure
A sequence of 11 enzymatic reactions results in the synthesis of the complete GPI anchor consisting of a hydrophobic and a hydrophilic portion. The hydrophobic portion comprises phosphatidylinositol, while the hydrophilic part comprises polar groups like phosphoethanolamine,...

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Related Experiment Video

Updated: Jun 24, 2026

Measuring the Rate of Lipolysis in Ex Vivo Murine Adipose Tissue and Primary Preadipocytes Differentiated In Vitro
09:41

Measuring the Rate of Lipolysis in Ex Vivo Murine Adipose Tissue and Primary Preadipocytes Differentiated In Vitro

Published on: March 17, 2023

GPIHBP1 and lipolysis: an update.

Anne P Beigneux1, Michael M Weinstein, Brandon S J Davies

  • 1Department of Medicine, David Geffen School of Medicine, University of California, Los Angeles, California, USA. abeigneux@mednet.ucla.edu

Current Opinion in Lipidology
|April 17, 2009
PubMed
Summary
This summary is machine-generated.

Glycosylphosphatidylinositol-anchored protein 1 (GPIHBP1) binds lipoprotein lipase (LPL), which is essential for processing triglyceride-rich lipoproteins. Its absence causes severe hypertriglyceridemia.

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Measurement of Basal and Forskolin-stimulated Lipolysis in Inguinal Adipose Fat Pads
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Measurement of Basal and Forskolin-stimulated Lipolysis in Inguinal Adipose Fat Pads

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Last Updated: Jun 24, 2026

Measuring the Rate of Lipolysis in Ex Vivo Murine Adipose Tissue and Primary Preadipocytes Differentiated In Vitro
09:41

Measuring the Rate of Lipolysis in Ex Vivo Murine Adipose Tissue and Primary Preadipocytes Differentiated In Vitro

Published on: March 17, 2023

Measurement of Basal and Forskolin-stimulated Lipolysis in Inguinal Adipose Fat Pads
07:59

Measurement of Basal and Forskolin-stimulated Lipolysis in Inguinal Adipose Fat Pads

Published on: July 21, 2017

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Background:

  • Triglyceride-rich lipoproteins (TRLs) are crucial for lipid transport.
  • Lipoprotein lipase (LPL) is the primary enzyme responsible for TRL hydrolysis.
  • GPIHBP1 is an endothelial cell-surface glycoprotein involved in lipid metabolism.

Purpose of the Study:

  • To review the structure of GPIHBP1.
  • To elucidate the role of GPIHBP1 in the lipolytic processing of TRLs.

Main Methods:

  • Analysis of Gpihbp1 knockout mice.
  • Cellular transfection studies with GPIHBP1 expression vectors.
  • Investigation of GPIHBP1 binding domains for LPL and chylomicrons.

Main Results:

  • Gpihbp1 knockout mice exhibit severe hypertriglyceridemia and milky plasma.
  • GPIHBP1 localizes to endothelial cells in tissues critical for lipolysis.
  • GPIHBP1 directly binds LPL and chylomicrons, with specific domains mediating LPL interaction.
  • Expression of GPIHBP1 is modulated by fasting/refeeding and PPAR-gamma.

Conclusions:

  • GPIHBP1 is an essential endothelial cell-surface protein for LPL binding and TRL lipolysis.
  • Deficiency in GPIHBP1 leads to impaired lipid processing and hypertriglyceridemia.