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Related Concept Videos

Telomeres and Telomerase02:41

Telomeres and Telomerase

In eukaryotic DNA replication, a single-stranded DNA fragment remains at the end of a chromosome after the removal of the final primer. This section of DNA cannot be replicated in the same manner as the rest of the strand because there is no 3’ end to which the newly synthesized DNA can attach. This non-replicated fragment results in gradual loss of the chromosomal DNA during each cell duplication. Additionally, it can induce a DNA damage response by enzymes that recognize single-stranded DNA.
Telomeres and Telomerase02:41

Telomeres and Telomerase

In eukaryotic DNA replication, a single-stranded DNA fragment remains at the end of a chromosome after the removal of the final primer. This section of DNA cannot be replicated in the same manner as the rest of the strand because there is no 3’ end to which the newly synthesized DNA can attach. This non-replicated fragment results in gradual loss of the chromosomal DNA during each cell duplication. Additionally, it can induce a DNA damage response by enzymes that recognize single-stranded DNA.
Single-Strand DNA Binding Proteins01:03

Single-Strand DNA Binding Proteins

For successful DNA replication, the unwinding of double-stranded DNA must be accompanied by stabilization and protection of the separated single strands of the DNA. This crucial task is performed by single-strand DNA-binding (SSB) proteins. They bind to the DNA in a sequence-independent manner, which means that the nitrogenous bases of the DNA need not be present in a specific order for binding of SSB proteins to it. The binding of SSB proteins straightens single-stranded DNA (ssDNA) and makes...
Restarting Stalled Replication Forks02:37

Restarting Stalled Replication Forks

DNA replication is initiated at sites containing predefined DNA sequences known as origins of replication. DNA is unwound at these sites by the minichromosome maintenance (MCM) helicase and other factors such as Cdc45 and the associated GINS complex.The unwound single strands are protected by replication protein A (RPA) until DNA polymerase starts synthesizing DNA at the 5’ end of the strand in the same direction as the replication fork. To prevent the replication fork from falling apart, a...
Replication in Eukaryotes01:29

Replication in Eukaryotes

In eukaryotic cells, DNA replication is highly conserved and tightly regulated. Multiple linear chromosomes must be duplicated with high fidelity before cell division, so there are many proteins that fulfill specialized roles in the replication process. Replication occurs in three phases: initiation, elongation, and termination, and ends with two complete sets of chromosomes in the nucleus.
Many Proteins Orchestrate Replication at the Origin
Eukaryotic replication follows many of the same...
Replication in Eukaryotes02:31

Replication in Eukaryotes

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Related Experiment Video

Updated: Jun 24, 2026

Analyzing Telomeric Protein-DNA Interactions Using Single-Molecule Magnetic Tweezers
11:21

Analyzing Telomeric Protein-DNA Interactions Using Single-Molecule Magnetic Tweezers

Published on: August 30, 2024

Sua5p a single-stranded telomeric DNA-binding protein facilitates telomere replication.

Fei-Long Meng1, Yan Hu, Ning Shen

  • 1The State Key Laboratory of Molecular Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Graduate School of the Chinese Academy of Sciences, Shanghai, China.

The EMBO Journal
|April 17, 2009
PubMed
Summary

Sua5p is a novel protein that binds single-stranded telomeric DNA and is essential for maintaining telomere length in Saccharomyces cerevisiae. This discovery adds to our understanding of telomere replication and maintenance.

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Last Updated: Jun 24, 2026

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Generation of Cancer Cell Clones to Visualize Telomeric Repeat-containing RNA TERRA Expressed from a Single Telomere in Living Cells
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Published on: January 17, 2019

Area of Science:

  • Molecular Biology
  • Genetics
  • Biochemistry

Background:

  • Telomere length maintenance in Saccharomyces cerevisiae involves a complex network of over 280 genes.
  • Previously essential genes are now being identified as crucial for telomere maintenance.

Purpose of the Study:

  • To identify novel genes involved in telomere maintenance.
  • To investigate the specific role of Sua5p in telomere replication and length regulation.

Main Methods:

  • Epistasis analysis and telomere sequencing in sua5Delta mutant yeast cells.
  • Biochemical, structural, and genetic studies to analyze Sua5p function.
  • In vitro binding assays to assess Sua5p interaction with telomeric DNA.

Main Results:

  • Sua5p specifically binds single-stranded telomeric (ssTG) DNA via a distinct surface region.
  • The DNA-binding ability of Sua5p is critical for its telomere maintenance function.
  • Sua5p acts downstream of telomerase recruitment and positively regulates telomere length in vivo.

Conclusions:

  • Sua5p is a novel single-stranded telomeric DNA-binding protein.
  • Sua5p plays a significant role in regulating telomere length in budding yeast.
  • This finding expands the known mechanisms of telomere replication and maintenance.